家兔血液灌流动物模型的构建和安全性评价

发布时间：2018-04-26 08:52

本文选题：血液灌流 + 动物模型　；参考：《中国血液净化》2017年03期

【摘要】：目的建立经家兔外周血管进行血液灌流(hemoperfusion,HP)的实验动物模型并对其安全性进行评价。方法 12只健康白色新西兰家兔随机分为2组,每组6只。HP组实验动物在表面麻醉下一侧耳中央动脉置入动脉留置针,另一侧耳缘静脉置入静脉留置针,动/静脉留置针依次通过管路连接恒流泵、血液灌流器、排空气管和三通阀构成HP体外循环,以恒流泵流速2m L/min进行HP持续2h。对照组除不连接血液灌流器外,按照HP组的操作进行体外循环。HP进行期间监测家兔的生命体征,在HP开始前和HP开始后2h、4h、8h、24h,采集两组动物外周血,检测血常规、血清蛋白(总蛋白、白蛋白和球蛋白)和电解质(Na~+、K~+、Ca~(2+)和Cl~-),评价该HP动物模型的安全性。结果 HP进行期间动物生命体征平稳,HP血流顺畅,实验期间未见动物死亡。HP组与对照组家兔红细胞计数(F=0.386,P=0.548)、平均红细胞体积(F=0.041,P=0.844)、血红蛋白(F=0.375,P=0.554)、白细胞计数(F=0.005,P=0.943)、淋巴细胞计数(F=0.212,P=0.655)、单核细胞计数(F=0.508,P=0.492)、中性粒细胞计数(F=0.026,P=0.875)、血小板计数(F=1.270,P=0.286)、平均血小板体积(F=0.037,P=0.851)、Na~+(F=1.012,P=0.338)、K~+(F=0.001,P=0.972)、Ca~(2+)(F=1.812,P=0.208)、血清总蛋白(F=4.929,P=0.051)和球蛋白水平(F=2.641,P=0.135)差别无统计学意义。HP组家兔血清白蛋白水平降低(F=27.490,P=0.000),Cl-水平升高(F=11.040,P=0.008),相对对照组差别有统计学意义。结论成功建立经家兔外周血管进行HP的实验动物模型,该模型安全可靠,可以满足HP实验需要。
[Abstract]:Objective to establish an experimental animal model of hemoperfusionus hemoperfusionus (HPP) via peripheral blood vessels of rabbits and to evaluate its safety. Methods Twelve healthy white New Zealand rabbits were randomly divided into two groups: 6 rabbits in each group. The experimental animals in HP group were placed with a central lateral auricular artery needle under surface anesthesia and vein indwelling needle with the other side of the auricular vein. The artery / vein indwelling needle was connected with the constant flow pump, the hemoperfusion device, the exhaust pipe and the three-way valve to form HP cardiopulmonary bypass (CPB). HP was carried out for 2 h with the constant flow pump flow rate (2m L/min). In the control group, the vital signs of rabbits were monitored during cardiopulmonary bypass (CPB) according to the operation of HP group. The vital signs of rabbits were monitored before and 2 hours after HP began. The peripheral blood was collected from the two groups and the blood routine was detected. Serum protein (total protein, albumin and globulin) and electrolyte Na ~ (+) K ~ (+) ~ (2) and Cl _ (2) were used to evaluate the safety of the HP animal model. Results during the period of HP, the vital signs of the animals were stable and the blood flow of HP was smooth. During the experiment, there was no animal death. HP group and control group, the red blood cell count of rabbits was 0.386, the mean volume of red blood cell was 0.041, the hemoglobin was 0.375, the white blood cell count was 0.005, the count of lymphocyte was 0.212, the count of monocyte was 0.508, the count of neutrophils was 0.8026, the count of platelets was 0.875, the count of leukocyte was 0.212P0. 655, the count of mononuclear cells was 0.508, the count of neutrophil was 0. 026, the count of platelets was 0. 875. The average platelet volume was 0.286m, and the mean platelet volume was 0.037P0.851. the serum albumin level of the HP group decreased F27.490P0.000Cl-increased F11.040P0.208, the serum total protein F4.929P0.051) and the serum albumin level F2.641P0.135. There was no statistical difference between the HP group and the control group. The serum albumin level of the HP group decreased F27.490P0.000Cl- the increase of the level of F27.490P0.000Cl- increased the level of F11.040P80.There was significant difference between the control group and the control group. Conclusion the experimental animal model of HP via rabbit peripheral blood vessels was established successfully. The model is safe and reliable and can meet the need of HP experiment.
【作者单位】：深圳市职业病防治院中毒控制中心;深圳市职业病防治院临床检验科;中山大学公共卫生学院;
【基金】：国家自然科学基金(81302460) 深圳市科创委课题(JCYJ20140414110951757)
【分类号】：R-332;R459.5

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