芒硝外敷预防留置针静脉治疗所致机械性静脉炎的实验研究

发布时间：2018-04-26 16:59

  本文选题：留置针 + 静脉炎　； 参考：《锦州医科大学》2017年硕士论文

【摘要】：目的本课题应用静脉留置针所致机械性静脉炎动物模型,观察芒硝外敷对静脉炎血管损伤及静脉血管组织中白介素-1(IL-1)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)表达的干预作用,探讨芒硝外敷对静脉留置针所致机械性静脉炎的预防作用,为预防静脉留置针所引起的机械性静脉炎提供实验数据。方法选取同种健康大耳新西兰兔57只,随机分为3组,分别为空白对照组(9只)、留置针组(24只)、芒硝外敷组(24只),留置针组和芒硝外敷组每只兔子的耳缘静脉进行静脉留置针穿刺。留置针组,每日一次进行静脉注射0.9%氯化钠注射液20ml;芒硝外敷组是在留置针组的基础上给予芒硝固定外敷。在留置的第3d,5d,7d时,空白对照组随机抽取3只,取耳缘静脉标本;留置针组和芒硝外敷组,分别随机抽取8只,拔除静脉留置针,取耳缘静脉标本,所有静脉标本做病理组织切片。光镜观察每组兔耳缘静脉HE染色病理组织切片,用免疫荧光法检测每组兔耳缘静脉血管组织中IL-1、IL-6、TNF-α的表达情况。结果HE染色结果:空白对照组无炎性细胞浸润,血管壁周围无纤维组织增生。随着留置时间的增加,留置针组及芒硝外敷组兔耳缘静脉均有血管壁纤维组织增生、血管组织中炎性细胞浸润增加、机化血栓增多。将芒硝外敷组与留置针组比较,血管壁中纤维组织明显降低,炎性细胞浸润显著减少。免疫荧光检测结果:在同一留置时间,留置针组和芒硝外敷组IL-1、IL-6、TNF-α表达均明显高于空白对照组(P0.05);芒硝外敷组IL-1、IL-6、TNF-α表达均低于留置针组(P0.05);留置针组和芒硝外敷组同组中不同时间比较,IL-1、IL-6、TNF-α的表达与留置时间呈正相关。结论芒硝外敷能明显减少兔耳缘静脉炎症细胞浸润、血管壁纤维组织增生及血栓形成。芒硝外敷可显著减少静脉留置针所致机械性静脉炎组织中IL-1、IL-6及TNF-α的表达,降低兔耳缘静脉的炎症反应。
[Abstract]:Objective to observe the effect of external application of glauberite on phlebitis vascular injury and the expression of interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-伪 (TNF- 伪) in vascular tissue of phlebitis by using the animal model of mechanical phlebitis induced by intravenous indwelling needle. To explore the preventive effect of external application of glauberite on mechanical phlebitis caused by intravenous indwelling needle, and to provide experimental data for preventing mechanical phlebitis caused by intravenous indwelling needle. Methods 57 healthy New Zealand rabbits were randomly divided into 3 groups. Nine rabbits in the blank control group, 24 rabbits in the indwelling needle group, 24 rabbits in the external application group of mirabilite, and each rabbit in the indwelling needle group and the external application group were punctured with vein indwelling needle. In the indwelling needle group, 0.9% sodium chloride injection 20ml was injected intravenously once a day, and the external application group was fixed on the basis of the indwelling needle group. At the 3rd day of indwelling for 5 days and 7 days after indwelling, 3 cases were randomly selected from the blank control group, 8 from the indwelling needle group and 8 from the external application group, respectively, the venous indwelling needle was extracted, and the auricular vein sample was taken from the auricular vein specimen. All the venous specimens were made by pathological sections. The pathological sections of rabbit auricular vein stained with HE were observed under light microscope, and the expression of IL-1 and IL-6 TNF- 伪 in vein tissue of each group was detected by immunofluorescence. Results HE staining showed that no inflammatory cells were infiltrated and no fibrous tissue proliferated around the vascular wall in the blank control group. With the increase of indwelling time, vascular wall fibrous tissue proliferation, inflammatory cell infiltration and organized thrombus increased in both the indwelling needle group and the external application group. Compared with the indwelling group, the fibrous tissue and inflammatory cell infiltration in the vascular wall were significantly decreased in the application group. Immunofluorescence results: at the same retention time, The expression of IL-1TNF- 伪 in the indwelling group and the external application group was significantly higher than that in the blank control group (P0.05A), and the expression of IL-1mil IL-6TNF- 伪 was lower than that in the indwelling needle group (P0.05A), and the expression of IL-1IL-6TNF- 伪 was positively correlated with the indwelling time in the indwelling needle group and the oral application group (P < 0.05). Conclusion Glauber's application can significantly reduce inflammatory cell infiltration, vascular wall fibrous tissue proliferation and thrombus formation in rabbit auricular vein. The expression of IL-1- 6 and TNF- 伪 in mechanical phlebitis tissue induced by intravenous indwelling needle was significantly reduced by external application of glauberite, and the inflammatory response of rabbit auricular vein was decreased.
【学位授予单位】：锦州医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R472

【相似文献】

相关期刊论文 前10条

1 龚蔚;罗月青;徐锦;方萌;;新型芒硝外敷袋的制作与应用[J];解放军护理杂志;2009年24期

2 宋小花;芒硝外敷治耳廓浆液性软骨膜炎验案[J];新中医;1997年01期

3 鲁玲娟,徐雪芬;大黄芒硝外敷结合频谱仪治疗妇产科术后切口愈合障碍[J];浙江中医学院学报;2003年06期

4 戴芙蓉,刘俊芬;芒硝外敷治疗产后乳房胀痛60例临床观察[J];中国民族民间医药杂志;2004年02期

5 李兰英,许焕芹;鲜芦荟芒硝外敷治疗损伤性肿胀[J];河南中医;2004年09期

6 李莉;;大黄、芒硝外敷辅助治疗下肢肿胀效果观察[J];齐鲁护理杂志;2006年16期

7 唐艳辉;杨秋华;李清清;;芒硝外敷治疗哺乳期乳房胀痛的效果观察[J];医学临床研究;2007年10期

8 刘彦;陈晖;孙晓芳;程英英;张真稳;朱妍;王晓敏;刘超;;芒硝外敷治疗糖尿病足的临床观察[J];河北中医;2009年08期

9 蒋丰慧;张厚宽;韩顺利;;芒硝外敷对剖宫产术后切口的影响观察[J];中国实用医药;2010年25期

10 周均;;芒硝外敷治疗卧床患者便秘效果观察[J];中国伤残医学;2010年06期

相关会议论文 前7条

1 王春芳;黄敏燕;;芒硝外敷联合超短波理疗促进小儿急性化脓性阑尾炎术后肠功能恢复的疗效观察[A];2013年浙江省医学会小儿外科学学术年会暨分会成立30周年庆典论文汇编[C];2013年

2 黄柳燕;沈麒云;李黎梅;;芒硝外敷对腹透病人下肢水肿影响的研究[A];全国第13届老年护理学术交流暨专题讲座会议、全国中医、中西医结合护理学术交流暨专题讲座会议论文汇编[C];2010年

3 周煜;雷聪云;叶永红;赵彩华;;芒硝外敷治疗重症急性胰腺炎腹胀效果观察[A];第三届浙江中西部科技论坛论文集（第八卷 护理分卷）[C];2006年

4 彭娟;马静;孙玉芝;;金黄散联合芒硝外敷糖尿病烧伤创面的作用研究[A];第十六次全国中西医结合疡科学术交流会论文汇编[C];2013年

5 李晓霞;李冬英;张华虹;;生大黄联合芒硝外敷治疗重症急性胰腺炎腹胀效果观察及护理[A];第8届全国重症监护专科护理新进展研讨会论文汇编[C];2011年

6 桂雨根;;生大黄灌胃、芒硝外敷治疗急性水肿型胰腺炎临床研究[A];中华医学会第七次全国消化病学术会议论文汇编（上册）[C];2007年

7 胡岗;蒋启梅;付华;吴军;吴华;;冰片、芒硝外敷配合综合疗法治疗重症急性胰腺炎疗效观察[A];贵州省中西医结合学会2005年消化系及肝病学术交流会论文集[C];2005年

相关重要报纸文章 前3条

1 魏开敏;大黄芒硝外敷可促手术伤口愈合[N];大众卫生报;2005年

2 浙江省玉环县中医院 王治宇 黄菊青;禹功散合芒硝外敷神阙治肝硬化[N];中国中医药报;2014年

3 衣晓峰 李华妍;冰片+芒硝外敷解除输液后红肿疼痛[N];健康报;2006年

相关硕士学位论文 前3条

1 李方;大黄灌胃灌肠联合芒硝外敷对重症急性胰腺炎临床疗效的Meta分析[D];湖北中医药大学;2016年

2 孙志霞;芒硝外敷对黄体酮肌注所致局部炎症疗效观察[D];河北医科大学;2016年

3 路燕燕;芒硝外敷预防留置针静脉治疗所致机械性静脉炎的实验研究[D];锦州医科大学;2017年

，

本文编号：1806870