泛耐药铜绿假单胞菌湖北襄阳分离株获得性耐药元件研究

发布时间：2018-04-30 09:49

本文选题：铜绿假单胞菌 + 泛耐药　；参考：《中华医院感染学杂志》2017年19期

【摘要】：目的调查一组泛耐药的铜绿假单胞菌获得性耐药元件基因的携带情况及菌株间的亲缘关系。方法收集2015年1-12月医院住院患者痰液标本分离到的20株泛耐药的铜绿假单胞菌,采用K-B法测定9种抗菌药物的敏感性,采用聚合酶链反应(PCR)及序列分析的方法分析35种β-内酰胺类获得性耐药基因与膜孔蛋白oprD2基因、21种氨基糖苷类获得性耐药基因和9种可移动遗传元件遗传标记。阳性耐药基因测序后直接作BLAST比对,对57种获得性耐药元件基因与膜孔蛋白oprD2基因检测结果作样本聚类分析(UPGMA法)。结果 20株铜绿假单胞菌为泛耐药菌。20株菌有19株分别检出1~3种β-内酰胺酶基因,仅1株未检出β-内酰胺酶基因且膜孔蛋白oprD2基因缺失。20株菌均检出氨基糖苷类药物获得性耐药基因和可移动遗传元件遗传标记基因。样本聚类分析提示20株菌有聚集性且可分成A、B群。其中A群中有4个克隆播散,B群中有2个克隆播散。结论 20株泛耐药铜绿假单胞菌同时携带了β-内酰胺类药物获得性耐药基因、氨基糖苷类药物获得性耐药基因和可移动遗传元件遗传标记,是对β-内酰胺类和氨基糖苷类产生耐药的重要原因。本组菌检出的6个克隆高度疑似医院感染,同一克隆菌株携带相同基因。
[Abstract]:Objective to investigate the gene carriers of acquired drug resistance elements of Pseudomonas aeruginosa and the relationship between them. Methods from January to December 2015, 20 strains of pan-resistant Pseudomonas aeruginosa were isolated from sputum samples of hospital inpatients. The sensitivity of 9 antimicrobial agents was determined by K-B method. Polymerase chain reaction (PCR) and sequence analysis were used to analyze 35 尾 -lactam acquired drug resistance genes and 21 aminoglycoside acquired resistance genes and 9 transportable genetic element genetic markers. The positive drug resistance genes were compared with BLAST directly after sequencing. The results of 57 acquired drug resistance element genes and membrane porin oprD2 gene were analyzed by cluster analysis with UPGMA method. Results among the 20 strains of Pseudomonas aeruginosa, 19 strains were pan-resistant. Only one strain did not detect 尾 -lactamase gene and the membrane-porin oprD2 gene was absent. All the 20 strains detected aminoglycoside acquired drug resistance gene and transportable genetic element genetic marker gene. Cluster analysis showed that 20 strains of bacteria were clustered and could be divided into Agna B group. There are 4 clones in group A and 2 in group B. Conclusion 20 strains of pan-resistant Pseudomonas aeruginosa carry 尾 -lactam acquired drug resistance genes, aminoglycoside acquired drug resistance genes and transportable genetic element genetic markers. It is an important cause of resistance to 尾-lactams and aminoglycosides. The 6 clones were highly suspected of nosocomial infection, and the same cloned strains carried the same gene.
【作者单位】：湖北医药学院附属襄阳市第一人民医院检验科;宁波市第一医院检验科;
【基金】：浙江省中医药基金资助项目(2011ZB126)
【分类号】：R446.5

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