一株人血源猪链球菌的分离鉴定与毒力基因检测

发布时间：2018-05-04 16:26

本文选题：猪链球菌 + VITEK　；参考：《中国人兽共患病学报》2017年07期

【摘要】：目的对北京某医院就诊的一例疑似人感染猪链球菌病患者的血液来源菌株进行鉴定与毒力因子检测分析。方法将患者血液分离菌株接种哥伦比亚血琼脂平板,经革兰氏染色镜检、血清凝集试验、VITEK 2Compact微生物分析仪以及基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)检测后,再用PCR技术检测猪链球菌种特异性基因(16S rRNA)和猪链球菌2型特异性荚膜多糖编码基因(cps2J)以及多个毒力基因:溶菌酶释放相关蛋白(mrp)、溶血素(sly)、细胞外蛋白因子(ef)、谷氨酸脱氢酶(gdh)、纤粘连蛋白结合蛋白(fbps)、甘油醛-3-磷酸脱氢酶(gapdh)的编码基因和毒力相关基因orf2。结果传统细菌鉴定方法和蛋白质谱技术以及核酸检测方法将这株患者血源性细菌分离株鉴定为猪链球菌2型,该菌株的毒力基因cps2J、sly、ef、gdh、fbps、gapdh和orf2的PCR检测均为阳性,mrp基因为阴性。结论该株人血源细菌分离株为猪链球菌2型sly+/ef+/mrp强毒株。
[Abstract]:Objective to identify and detect virulence factors of a blood source strain from a suspected human infected Streptococcus suis patient in a hospital in Beijing. Methods the isolated strains were inoculated into Colombian blood Agar plate, examined by Gram staining, serum agglutination test (VITEK) microorganism analyzer and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS). PCR technique was used to detect the specific gene of Streptococcus suis (16s rRNAs) and type 2 specific capsular polysaccharide coding gene (CPS2J), and several virulence genes: lysozyme releasing associated protein, hemolysin lycin, extracellular protein factor (ECF), and other virulence genes. The coding gene and virulence related gene orf2 of aminic acid dehydrogenase, fibronectin binding protein, glyceraldehyde-3-phosphate dehydrogenase and glyceraldehyde-3-phosphate dehydrogenase Results the isolates were identified as Streptococcus suis type 2 by traditional methods of bacterial identification, protein spectrum technique and nucleic acid detection. The virulence genes of the strain, cps2J, slyefeng, fbpsgapdh and orf2, were both negative. Conclusion this strain is a virulent strain of Streptococcus suis type 2 sly / ef / MRP.
【作者单位】：北京市疾病预防控制中心北京市预防医学研究中心;首都医科大学公共卫生学院;
【基金】：国家科技重大专项(No.2012ZX10004215-003-001)资助~~
【分类号】：R446.5

【参考文献】