直接稀释-电感耦合等离子体质谱测定脐血清中22种元素

发布时间：2018-05-09 21:57

  本文选题：电感耦合等离子体质谱 + 血清　； 参考：《卫生研究》2017年05期

【摘要】：目的建立电感耦合等离子体质谱(inductively coupled plasma mass spectrometry,ICP-MS)测定人脐血清中22种元素含量的方法。方法吸取血清样本以2%硝酸15倍稀释,5000 r/min离心5 min,取上清液进样分析。用Ge作为内标元素校正八极杆碰撞模式定量元素(Fe、As、Se、I),用Sc、Ge、In、Bi作为标准模式定量元素的内标。ICP-MS射频功率1550 W,等离子气体流量15.00 L/min,进样量0.30 mL/min,驻留时间90 ms;八极杆反应气体为高纯He气,气体流速1.0 L/min,射频功率150 V,能量歧视5.0 V。结果内标标准曲线法定量,各元素在其线性范围内线性良好(r0.9993),检出限为0.00158~1.21μg/L。质控血清测得值在标准值范围内。方法用于实际血清测定,加标回收率在86.4%~116%,测定精密度在0.164%~18.79%(n=6)。结论 ICP-MS法快速、灵敏、准确,操作简单,能有效地检测脐血清中各元素含量。
[Abstract]:Objective to establish an inductively coupled plasma mass spectrometry (ICP-MS) method for the determination of 22 elements in human umbilical serum. Methods the serum samples were centrifuged with 2% nitric acid 15 times dilution of 5 000 r/min for 5 min and the supernatant was analyzed. Using GE as the internal standard element to correct the quantitative element of the ocpole collision mode, using ScGe Ge Inn Bi as the internal standard of the quantitative element of the standard mode, ICP-MS RF power 1550 W, plasma gas flow rate 15.00 L / min, injection amount 0.30 mL / min, residence time 90 Ms, and the reaction gas of the eight pole is high purity he gas, Gas flow rate 1.0 L / min, RF power 150 V, energy discrimination 5.0 V. Results the standard curve of internal standard was used to determine the quantity. The linear range of each element was good. The detection limit was 0.00158 ~ 1.21 渭 g 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) ~ (-1) ~ (-1). The value of quality control serum is in the range of standard value. The method has been applied to the determination of actual serum. The recovery rate of the added standard is 86.4 and the precision of the determination is 0.164 and 18.79. Conclusion ICP-MS method is rapid, sensitive, accurate and easy to operate, and can be used for the determination of all elements in umbilical serum.
【作者单位】： 四川大学华西公共卫生学院卫生检验与检疫系;四川大学华西第二医院出生缺陷中心;四川省疾病预防控制中心;
【基金】：十二五规划科学支撑计划项目(No.2014BAI06B01) 国家基础性工作专项(No.2014FY110700)
【分类号】：O657.63;R446.1
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