献血者HIV RNA窗口期标本的追踪和随访

发布时间：2018-05-11 22:18

本文选题：HIV窗口期 + 献血者追踪检测　；参考：《中国输血杂志》2017年06期

【摘要】：目的对处于HIV RNA窗口期的献血者做追踪、随访和验证。方法分别使用第3、4代酶联免疫试验(ELISA)方法(试剂)、免疫印迹试验(WB),以及核酸检测技术(NAT)定性和定量等方法,对在血液筛查中检出的1例献血者HIV窗口期不同时间(根据献血者的配合度间隔4-8 d)的血液标本做追踪和随访检测。采用ELISA方法以双试剂检测抗-HIV;同时采用全自动NAT单检分析系统,对该例血液标本做了HBV、HCV、HIV 3项NAT联检和鉴别试验。结果该例献血者标本首次检测:ELISA抗-HIV非反应性,NAT定性试验反应性;随后NAT定量试验和抗-HIV确认试验(WB)也同为阴性。采用双试剂ELISA和WB确认方法对献血者追踪、随访检测4次,至3周后确认为血清学抗-HIV转阳,获得到了1个完整的血清学转化结果。结论首次追踪、随访检测得以确认1例重庆地区病毒载量较高的HIV RNA窗口期献血者;NAT检测有利于阻截该HIV RNA窗口期献血者的血液流向临床。
[Abstract]:Objective to follow up, follow up and verify the blood donors in the window of HIV RNA. Methods qualitative and quantitative methods were used in the third and fourth generation enzyme linked immunosorbent assay (Elisa) (Reagent, Western blotting, and Nucleic acid detection), respectively. The blood samples of one blood donor at different time (4-8 days interval according to the donor's matching degree) in HIV window period detected by blood screening were tracked and followed up. The ELISA method was used to detect anti-HIVs and the automatic NAT single detection and analysis system was used to detect and identify the three items of NAT in the blood samples of this case. Results the blood donor sample was tested for the first time for the qualitative reaction of anti-HIV non-reactive Nat test, and then the NAT quantitative test and anti-HIV confirmation test were also negative. Double reagent ELISA and WB confirmations were used to track the blood donors. Four follow-up tests were carried out. After 3 weeks, the results were confirmed as serological anti-HIV positive, and a complete serological transformation result was obtained. Conclusion A case of HIV RNA blood donor with high viral load in Chongqing area can be confirmed by follow-up examination for the first time. The detection of Nat is beneficial to blocking the blood flow of the donor during the HIV RNA window period.
【作者单位】：重庆市血液中心;Chongqing
【分类号】：R446.62

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