基质辅助激光解析飞行质谱法检测空肠弯曲菌及李斯特菌

发布时间：2018-05-16 14:48

本文选题：VITEK + MS微生物检测系统　；参考：《食品科学》2017年20期

【摘要】：目的:通过VITEK MS微生物检测系统对养殖场及市售活鸡来源的空肠弯曲菌和单核细胞性李斯特菌进行激光解析飞行质谱鉴定,建立两种致病菌的质谱鉴定方法。方法:从样品中分离获得疑似的空肠弯曲菌和单核细胞性李斯特菌,经不同配比的基质液处理后进行质谱鉴定。通过分析检出菌特征峰及相对丰度差异来评价不同配比基质液对两种致病菌检测的影响。结果:空肠弯曲菌和李斯特菌对基质液配比要求具有明显差异,前者不需要经过甲酸裂解,而对三氟乙酸含量敏感,后者鉴定结果受到甲酸裂解制约。基质液含水量控制在体积分数30%可以有效避免特征峰缺失并提高检出率。结论:控制基质液的含水量、基质浓度和三氟乙酸含量可以增加空肠弯曲菌和李斯特菌的特征峰数量,增强相对丰度,提高致病菌检出率。基质液配比优化研究有助于增强食源性致病菌快速鉴定能力,有助于提高疾病预防和食源性风险监测的时效性。
[Abstract]:Objective: to identify Campylobacter jejuni and Listeria monocytogenes from poultry farms and live chickens by VITEK MS microbiological detection system, and to establish two methods for identification of pathogenic bacteria. Methods: the suspected Campylobacter jejuni and Listeria monocytogenes were isolated from the samples. By analyzing the characteristic peaks and the relative abundance differences, the effects of different proportion of matrix solution on the detection of two pathogenic bacteria were evaluated. Results: there was significant difference between Campylobacter jejuni and Listeria in the proportion of matrix solution. The former was sensitive to the content of trifluoroacetic acid without formic acid cracking, and the latter was restricted by formic acid cracking. Controlling the water content of matrix solution at 30% of volume fraction can effectively avoid the absence of characteristic peak and increase the detection rate. Conclusion: controlling the water content, the concentration of matrix and the content of trifluoroacetic acid can increase the number of characteristic peaks of Campylobacter jejuni and Listeria jejuni, enhance the relative abundance and increase the detection rate of pathogenic bacteria. The optimization of matrix ratio is helpful to enhance the ability of rapid identification of foodborne pathogens and to improve the timeliness of disease prevention and foodborne risk monitoring.
【作者单位】：常州市疾病预防控制中心;泰州医药高新技术产业园区疫苗工程中心;南京大学医学院;
【基金】：江苏省自然科学基金项目(BK20150250) 江苏省卫生计生委科研项目(Y2015016) 常州市科技项目(CE20165042)
【分类号】：R446.5

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