血液中分离一株O139群霍乱弧菌分子生物学检测及药物敏感性分析

发布时间：2018-05-21 21:28

本文选题：霍乱弧菌 + 毒力基因　；参考：《中国抗生素杂志》2017年01期

【摘要】：目的对南昌大学第一附属医院从患者血液中分离的一株O139群霍乱弧菌进行毒力基因检测、药物敏感试验及溯源分析。方法用PCR检测技术检测霍乱ctx A、ctx B、ace、cep、zot、rtx A、hly A、tox R、tcp A和cri毒力基因,用最小抑菌浓度(minimum inhibitory concen tration,MIC)进行体外抗菌药物敏感性检测,利用脉冲场凝胶电泳技术(pulsed-field gel electrophoresis,PFGE)进行分子同源性分析。结果 rtx A、hly A和tox R毒力基因为阳性,ctx A、ctx B、ace、cep、zot、tcp A和cri毒力基因均为阴性;药物敏感试验显示该菌对头孢吡肟、头孢曲松、头孢西丁、亚胺培南、强力霉素敏感,对氨苄西林、阿莫西林/克拉维酸、环丙沙星、庆大霉素、磺胺甲VA唑、复方磺胺甲VA唑和阿奇霉素耐药;PFGE分型显示与近5年江西省分离的O139群霍乱弧菌相似性聚类分析为80%。结论江西省首次在患者血液中分离出O139群霍乱弧菌,该菌对喹诺酮类、磺胺类和青霉素类药物具有较高的耐药性,PFGE溯源分析该菌与近5年江西省分离的O139群霍乱弧菌存在一定差异。
[Abstract]:Objective to detect virulence gene, drug sensitivity test and traceability analysis of Vibrio cholerae O139 isolated from patients' blood in the first affiliated Hospital of Nanchang University. Methods PCR was used to detect the virulence genes of Cholera ctx Bocacea cepceptor rtx Ahly Atox rtcp A and cri. The sensitivity of antimicrobial agents in vitro was detected by minimum inhibitory concentration (minimum inhibitory concen). The molecular homology was analyzed by pulsed-field gel electrophoresism using pulsed-field gel electrophoresis (PGE). Results the virulence genes of rtx Amohly A and tox R were all negative, and the drug sensitive tests showed that the virulence genes of rtx Amohly A and tox R were both negative to cefepime, ceftriaxone, cefoxitin, imipenem, doxycycline and ampicillin, and were sensitive to ampicillin. Amoxicillin / clavulanic acid, ciprofloxacin, gentamicin, sulfamethoxazole, compound sulfamethoxazole and azithromycin resistant PFGE showed that the similarity cluster analysis of O139 Vibrio cholerae isolated from Jiangxi Province in recent 5 years was 80%. Conclusion Vibrio cholerae O139 was isolated from patients' blood for the first time in Jiangxi Province. PFGE analysis showed that sulfonamides and penicillin had higher drug resistance. There were some differences between this strain and Vibrio cholerae O139 isolated in Jiangxi Province in recent 5 years.
【作者单位】：南昌大学第一附属医院检验科;江西省疾病预防控制中心传染病所;
【基金】：江西省科技厅项目基金(No.20151BBG70106)
【分类号】：R440

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