基于电化学原理的血红蛋白快速检测方法研究

发布时间：2018-06-02 08:55

本文选题：血红蛋白检测 + 电化学　；参考：《浙江大学》2017年硕士论文

【摘要】：血红蛋白是一种在人体内负责运载氧气的蛋白质。当氧含量高的时候,它表现出高氧相容性;氧含量低时,它就释放出结合的氧气。血红蛋白是表征失血性贫血、遗传性贫血和一些其他疾病,比如先天性心脏病、阻塞性肺气肿,的重要指标。而目前的血红蛋白传感器大多是基于光学原理制作的,光源不稳定,易被污染,易受环境干扰。本研究的目标就是基于丝网印刷碳糊电极和电化学方法制备一种低成本、一次性的血红蛋白传感器,并在此基础上将检测过程集成化,免去检测中额外的血液预处理过程。本文首先制备了一种需要溶血步骤的,基于丝网印刷碳糊电极的血红蛋白传感器。通过优化溶血素的选择和浓度、溶血振荡时间、电子传递剂用量以及工作参数,检测了最优工作条件下传感器的灵敏度、线性度、一致性和抗干扰性。实验结果表明,传感器的线性检测范围为20g/L-230g/L,灵敏度为0.033μA/(g/L),RSD小于4.59%,且抗干扰性强。在医院临床测试中,和医院标准仪器的相关性达到了 0.9536。本文还进一步研制了集成溶血和检测两部分的血红蛋白传感器。首先,选择了适合一步式溶血的溶血素,确定了溶血素的滴涂位置。之后制作了基于微流路和丝网印刷电极的血红蛋白传感器。在最优条件下,制备的传感器的线性检测范围为70 g/L-230 g/L,其灵敏度为0.041 μA/(g/L),具有较好的一致性。
[Abstract]:Hemoglobin is a protein responsible for carrying oxygen in the body. When oxygen content is high, it shows hyperoxia compatibility; when oxygen content is low, it releases the combined oxygen. Hemoglobin is an important indicator of hemorrhagic anemia, hereditary anemia and some other diseases, such as congenital heart disease, obstructive emphysema. At present, most of the hemoglobin sensors are based on the optical principle. The light source is unstable, easily polluted and easily disturbed by the environment. The aim of this study is to prepare a low cost, disposable hemoglobin sensor based on the screen printing carbon paste electrode and electrochemical method, and on this basis, the detection process is integrated and removed from inspection. A hemoglobin sensor, based on the screen printing carbon paste electrode, was first prepared. The sensitivity and linearity of the sensor were detected by optimizing the selection and concentration of the hemolysin, the time of the hemolysis oscillation, the amount of the electron transfer agent and the working parameters. The experimental results show that the linear detection range of the sensor is 20g/L-230g/L, the sensitivity is 0.033 A/ (g/L), the RSD is less than 4.59%, and the anti-interference ability is strong. In the clinical test of the hospital, the correlation with the standard instrument of the hospital has reached 0.9536. and the blood red egg with the integration of hemolysis and the detection of two parts is further developed. First, the hemolysin suitable for one step hemolysis is selected to determine the position of the hemolysin. Then the hemoglobin sensor based on the microfluidic and screen printing electrodes is produced. Under the optimal condition, the linear detection range of the sensor is 70 g/L-230 g/L, with a sensitivity of 0.041 mu A/ (g/L), which has a better one. Sex.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R446.1;TP212

【参考文献】