血培养病原菌分布及耐药分析

发布时间：2018-06-03 15:31

本文选题：血培养 + 病原菌　；参考：《中国医学创新》2017年10期

【摘要】：目的:分析包头医学院第二附属医院2015年1月-2016年11月血培养中病原菌分布及其对抗菌药物的敏感情况,为临床合理使用抗菌药物提供依据。方法:采用美国BD公司的BACTEC 9050全自动血培养仪,对血培养瓶进行连续培养监测,BD公司生产的Phoenix 100系统全自动微生物分析系统对血标本中分离的病原菌进行鉴定和药物敏感试验,利用WHONET 5.6软件来对药敏结果进行分析。结果:1307份血培养标本中,病原菌的分离数量共182株,阳性检出率为13.9%,其中革兰阴性菌120株,占65.9%;革兰阳性菌61株,占33.5%;真菌1株,占0.6%。对大肠埃希菌、克雷伯菌属、葡萄球菌属、肠球菌属进行药敏结果分析,葡萄球菌属对万古霉素、利奈唑胺100%敏感,大肠埃希菌以及克雷伯菌属对亚胺培南和美罗培南100%敏感,其他抗菌药物则有不同程度的耐药。结论:目前本院血培养分离出的病原菌主要以革兰阴性菌为主,菌种多样化,且呈现较高的耐药率,提示临床医生应重视血培养,以便合理应用抗菌药物。
[Abstract]:Objective: to analyze the distribution of pathogenic bacteria and its susceptibility to antibiotics in the second affiliated Hospital of Baotou Medical College from January 2015 to November 2016 so as to provide evidence for rational use of antibiotics in clinical practice. Methods: the BACTEC 9050 automatic blood culture instrument of BD Company was used to monitor the continuous culture of the blood culture bottle. The Phoenix 100 system automatic microbiological analysis system produced by BD Company was used to identify the pathogenic bacteria isolated from the blood samples and to test their drug sensitivity. The results of drug sensitivity were analyzed by WHONET 5.6 software. Results 182 strains of pathogenic bacteria were isolated from 1 07 blood samples, and the positive rate was 13. 9%, of which 120 were Gram-negative bacteria (65. 9%), 61 were Gram-positive bacteria (33. 5%) and 1 was fungi (0. 6%). The susceptibility of Escherichia coli, Klebsiella, Staphylococcus and Enterococcus to vancomycin and linazolamine was 100%, and Escherichia coli and Klebsiella were 100% sensitive to imipenem and meropenem. Other antimicrobial agents have varying degrees of resistance. Conclusion: the main pathogenic bacteria isolated from blood culture in our hospital at present are Gram-negative bacteria, variety of bacteria species and high drug resistance rate, suggesting that clinicians should pay attention to blood culture in order to use antibiotics rationally.
【作者单位】：包头医学院第二附属医院;
【分类号】：R446.5

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