云南楚雄地区彝族献血人群KIR基因多态性研究

发布时间：2018-06-05 02:06

  本文选题：杀伤细胞免疫球蛋白样受体 + 云南彝族　； 参考：《中国输血杂志》2017年10期

【摘要】：目的研究云南楚雄地区彝族献血人群KIR基因及基因型多态性特点。方法从云南地区无偿献血者中选取157名楚雄彝族健康自愿者,采用多重PCR-SSP方法进行KIR基因检测。根据http://www.allelefrequencies.net/中的ID号命名基因型和单体型组成。结果在云南地区楚雄彝族人群中,框架基因KIR3DL2、3DL3、2DL4和3DP1存在于所有个体;较为常见KIR基因有2DP1、2DL1、2DL3、3DL1和2DS4,其基因频率分别为0.920 2、0.920 2、0.861 8、0.774 3和0.760 6;频率较低的基因有KIR 2DS3、2DS2、2DL2、2DS5、3DS1、2DS1和2DL5,其基因频率分别为0.097 1、0.140 4、0.144 1、0.182 2、0.226 2、0.226 2和0.277 3;共发现19种KIR基因型,其中以ID号为1和2的基因型最为常见,频率为47.13%和16.56%。结论云南地区楚雄彝族人群KIR基因分布与中国南方汉族人群之间较一致,部分基因位点与韩国人、南非人,乌鲁木齐地区维吾尔族人和高加索地区人差异显著。
[Abstract]:Objective to study the polymorphism of KIR gene and genotype in Yi nationality in Chuxiong, Yunnan. Methods 157 healthy volunteers of Chuxiong Yi nationality were selected from volunteer blood donors in Yunnan area. The KIR gene was detected by multiple PCR-SSP method. The genotypes and haplotypes were named according to the ID number in http://www.allelefrequencies.net/. Results the frame genes KIR3DL2O3DL3DL4 and 3DP1 were found in all individuals in Chuxiong Yi nationality in Yunnan. The more common KIR genes were 2DP1DL1O2DL3DL1 and 2DS4, with the gene frequencies of 0.920 20.920 2U 0.861 80.774 3 and 0.760 6, respectively. The lower frequency genes were KIR 2DS 2DS 2DS 1DS 1DS 2DS 1DS 2DS 1 and 2DL5, the frequencies of which were 0.097 1T 0.140 40.144 0.182 2G 0.2226 20.226 2226 and 0.277 33.19 KIR genotypes were found. The genotypes with ID numbers 1 and 2 were the most common, with frequencies of 47.13% and 16.56%. Conclusion the distribution of KIR gene in Chuxiong Yi population in Yunnan is consistent with that of Han nationality in southern China. Some of the gene loci are significantly different from Korean, South African, Uygur and Caucasian people in Urumqi.
【作者单位】： 中国医学科学院北京协和医学院输血研究所;云南昆明血液中心;
【基金】：昆明市科技计划重点项目(昆科计字2013-04-02-AS-01-2141号) 中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-024)
【分类号】：R394
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本文编号：1979938