亚抑菌浓度庆大霉素对铜绿假单胞菌的影响

发布时间：2018-06-13 01:27

本文选题：庆大霉素 + 压抑菌浓度　；参考：《中国现代医学杂志》2017年26期

【摘要】：目的探讨亚抑菌浓度庆大霉素(GEN)对铜绿假单胞菌(PAO1)浮游菌和生物膜的影响。方法采用微量肉汤稀释法检测PAO1对GEN的敏感性。采用96孔板比浊法检测亚抑菌浓度GEN对PAO1浮游菌生长的影响,并通过结晶紫染色法检测亚抑菌浓度GEN对PAO1生物膜形成的影响。采用化学反应比色法测定亚抑菌浓度GEN对PAO1毒力因子表达的影响。结果 GEN对PAO1的最低抑菌浓度为2μg/ml;而当GEN浓度≤0.250μg/ml时,不影响PAO1浮游菌的增殖;当GEN为0.125μg/ml时,能促进PAO1生物膜的形成(P0.05),且在一定范围内(0.125~0.250μg/ml)随着GEN浓度增高,其促进生物膜形成的能力越强;0.125和0.250μg/ml GEN能促进群体密度信号系统相关基因las I、las R、rhl I、rhl R、pqs C、pqs D和毒力因子青脓素、弹性蛋白酶、碱性蛋白酶的表达(P0.05),且呈一定的剂量依赖性。结论亚抑菌浓度GEN在一定浓度范围内能促进PAO1生物膜的形成,并进一步促进群体密度信号系统相关基因和毒力因子的表达。
[Abstract]:Objective to investigate the effects of gentamicin on phytoplankton and biofilm of Pseudomonas aeruginosa (PAO1). Methods the sensitivity of PAO1 to Gen was detected by broth dilution method. The effect of sub inhibitory concentration Gen on the growth of PAO1 phytoplankton was detected by 96 well plate turbidimetry, and the effect of sub inhibitory concentration Gen on the formation of PAO1 biofilm was detected by crystal violet staining. The effect of sub inhibitory concentration Gen on the expression of PAO1 virulence factor was determined by chemical reaction colorimetry. Results the minimum inhibitory concentration of Gen on PAO1 was 2 渭 g / ml, but when Gen concentration was less than 0.250 渭 g/ml, it did not affect the proliferation of PAO1, when Gen was 0.125 渭 g/ml, it could promote the formation of PAO1 biofilm (P0.05N), and the concentration of Gen was 0.1250.250 渭 g / ml. The stronger its ability to promote biofilm formation, the more 0.125 and 0.250 渭 g/ml Gen could promote the expression of population density signaling system related gene (las) and the expression of virulence factor cypus, elastase and alkaline protease in a dose-dependent manner, in a dose-dependent manner. Conclusion Gen can promote the formation of PAO1 biofilm and further promote the expression of genes and virulence factors related to population density signaling system.
【作者单位】：贵州盘江投资控股(集团)有限公司总医院检验科;
【分类号】：R446.5

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