自制石墨烯—二硫化钼—全氟磺酸修饰的玻碳电极作为电化学生物传感器检测人血清尿酸

发布时间：2018-06-17 06:41

本文选题：尿酸 + 石墨烯　；参考：《川北医学院》2017年硕士论文

【摘要】：目的:目前用于检测尿酸(uric acid,UA)的大部分方法尚不能同时满足快速准确、高灵敏度、高特异性、检测线性范围宽、费用低廉等要求。通过对自然界其他多种生物或化学成分的检测表明,电化学法是最有可能同时满足上述要求的检测方法,而运用这种方法存在的主要难点在于寻找针对被检测物质的理想电极材料。本研究的目的,旨在为检测尿酸找到一种同时具有灵敏度高、检测速度快和线性范围宽等特点的优良电极感应材料。方法:在本研究中,我们制备了一种用石墨烯-二硫化钼-全氟磺酸复合物修饰的玻碳电极(G-MoS2-Nafion/GCE)作为检测尿酸的工作电极,并利用扫描电子显微镜(scanning electron microscopy,SEM)、元素面分布能谱(elemental distribution spectrometry,EDS)和X线衍射仪(X-ray diffraction,XRD)对G-MoS2复合物的形貌和元素组成进行表征分析。利用循环伏安法(cyclic voltammetry,CV)、线性扫描伏安法(linear sweep voltammetry,LSV)和计时电流法(amperometric i-t curve,i-t)对UA在G-MoS2-Nafion/GCE电极上的电化学行为。然后后,对电化学法检测UA是否会受到葡萄糖、抗坏血酸、多巴胺等物质的干扰,以及本研究中的电化学方法的准确度和精密度进行了综合分析。结果:本研究结果表明:(1)在对人体血清样本的分析中只探测到UA还原峰,说明本研究所建立的方法对人体血清UA的测定具有较高的特异性;(2)UA浓度与其对应的电流强度呈线性关系(拟合曲线为y=0.012x+0.645,线性拟合度R2=0.998),且具有较宽的线性范围和较高的灵敏度(最低检出限3S/N=13.91μM);(3)本方法测得人体血清UA的浓度与自动生化分析仪尿酸酶法测得的值具有良好的线性关系(拟合曲线为y=0.9802x+11.494,线性拟合度R2=0.978);(4)本研究采用的电化学法检测UA的平均回收率达95.28%,表明本研究中采用的方法对UA的检测具有较高的准确度;(5)重复性试验进一步表明,本研究中的用于检测UA的电化学方法还具有较高的精密度。结论:经本研究证实,利用G-MoS2-Nafion/GCE作为工作电极检测人血清UA的电化学方法具有灵敏度高、线性范围宽、检测速度快、抗干扰能力强,以及制作成本低、准确度和重复性较好等优势。因此,将G-MoS2-Nafion用于检测人血清UA浓度具有较好的临床应用前景。
[Abstract]:Objective: at present, most of the methods used in the detection of uric acid acidate UAA can not meet the requirements of rapid, accurate, high sensitivity, high specificity, wide linear range and low cost. The detection of many other biological or chemical components in nature shows that electrochemical method is the most likely to meet the above requirements. The main difficulty in using this method is to find the ideal electrode material for the detected substance. The aim of this study is to find an excellent electrode sensing material with high sensitivity, high detection speed and wide linear range for the detection of uric acid. Methods: in this study, we prepared a glassy carbon electrode modified with graphene, molybdenum disulfide and perfluorosulfonic acid complex, G-MoS2-Nafion / GCE, as the working electrode for the detection of uric acid. The morphology and elemental composition of G-MoS2 complex were characterized by scanning electron microscopy (SEM) and scanning electron microscopyscine (SEM), elemental distribution spectroscopy (EDS) and X-ray diffractometry (XRD). The electrochemical behavior of UA at G-MoS2-Nafion / GCE electrode was investigated by cyclic voltammetry, linear sweep voltammetry (LSVV) and chronoamperometric method. After that, the accuracy and precision of the electrochemical method for the detection of UA were analyzed comprehensively, including the interference of glucose, ascorbic acid, dopamine and so on, as well as the accuracy and precision of the electrochemical method in this study. Results: the results showed that only UA reduction peak was detected in the analysis of human serum samples. The results show that the method established in this paper has a high specificity for the determination of UA in human serum and has a linear relationship with the corresponding current intensity (the fitting curve is yr 0.012x 0.645, the linearity is R20.998m), and the linear range is wide. Higher sensitivity (minimum detection limit 3s / N = 13.91 渭 m ~ (-1) this method has a good linear relationship between the concentration of UA in human serum and the value measured by uric acid enzyme method of automatic biochemical analyzer (fitting curve is 0.9802x 11.494, linearity is R _ 2O _ 2 ~ (0.9784). The average recovery rate of the electrochemical method for the detection of UA is 95.28%, which indicates that the method used in this study has high accuracy in the determination of UA. The electrochemical method used to detect UA in this study also has high precision. Conclusion: the electrochemical method using G-MoS2-Nafion / GCE as working electrode for the detection of human serum UA has the advantages of high sensitivity, wide linear range, fast detection speed, strong anti-interference ability and low production cost. Good accuracy and repeatability. Therefore, G-MoS2-Nafion has a good clinical application prospect in the detection of human serum UA concentration.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R446.1;TP212.3

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