深慢波睡眠剥夺对大鼠睾丸组织氧化应激反应的影响

发布时间：2018-06-21 21:57

本文选题：睡眠时相剥夺 + 小平台水环境　；参考：《中华男科学杂志》2017年08期

【摘要】：目的:研究深慢波睡眠剥夺对大鼠睾丸组织氧化应激反应的影响。方法:36只5周龄健康雄性Wistar大鼠,随机分为深慢波睡眠时相剥夺组(SD1组)、深慢波睡眠时相及时长剥夺组(SD2组)和空白对照组(CC组),每组12只,利用小平台水环境建立深慢波睡眠剥夺模型。SD1组每间隔24 min干扰1次,SD2组每间隔24min剥夺睡眠8 min;夜间都进行12 h的完全睡眠剥夺。CC组模拟正常的12 h光照、12 h黑暗时间。28 d后,大鼠股动脉放血,留取睾丸组织进行称重,测定睾丸组织中蛋白含量、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物岐化酶(SOD)水平,显微镜下观察睾丸病理结构的改变。结果:SD1、SD2组大鼠的终末体质量[(248.1±25.1)g、(232.9±10.1)g]均下降,与CC组[(268.5±1.6)g]相比差异有统计学意义(P均0.05);与CC组相比,SD2组睾丸相对质量明显升高[(54.9±3.5)×10~(-2)vs(50.0±1.3)×10~(-2),P0.05]。与CC组相比,SD2组睾丸组织蛋白含量、MDA含量、SOD活性和GSH-Px均有显著差异[蛋白含量:(4.5±0.9)g pro/L vs 6.3±1.4)g pro/L;MDA含量:(1.3±0.3)nmol/mg pro vs(1.1±0.1)nmol/mg pro;SOD活性:(135.2±26.9)U/mg pro vs(104.3±33.1)U/mg pro;GSH-Px活性:(21.7±4.3)U/mg pro vs(15.6±4.0)U/mg pro,P均0.05],而与SD1组比较差异无统计学意义。SD1组睾丸病理学改变不明显,但SD2组睾丸生精小管管腔变小,周围间质部分增加,间质充血水肿明显。结论:长期深慢波睡眠剥夺对大鼠睾丸组织结构产生损伤并引起氧化应激反应。
[Abstract]:Aim: to study the effects of deep slow wave sleep deprivation on oxidative stress in rat testis. Methods Thirty-six five-week-old male Wistar rats were randomly divided into deep slow wave sleep deprivation group (SD1 group), deep slow wave sleep phase deprivation group (SD2 group) and control group (CC group) with 12 rats in each group. The model of deep slow wave sleep deprivation was established by using a small platform water environment. SD1 group was deprived of sleep by 24min for 8 minutes every 24 min disturbance per interval, and 12 hours of complete sleep deprivation. CC group simulated normal 12 h illumination for 12 h and darkness time of 12 h. 28 d later, SD2 group was deprived of sleep 8 minutes per interval. The rat femoral artery was bled and the testis were taken for weighing. The contents of protein, malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in testis were measured. The pathological structure of testis was observed under microscope. Results compared with CC group [(268.5 卤1.60) g], the end body weight of SD _ 2 group [(248.1 卤25.1) g, () 232.9 卤10.10 g] was significantly lower than that of control group (P < 0.05), and the testis relative weight of SD _ 2 group was significantly higher than that of CC group [(54.9 卤3.5) 脳 10 ~ (-2) vs (-2) vs () 脳 10 ~ (-2) P 0.05]. Compared with CC group, SD2 group had significant difference in testicular tissue protein content, SOD activity and GSH-Px. [protein content: (4.5 卤0.9) g / L vs 6.3 卤1.4 g / L: (1.3 卤0.3) nmol/mg pro vs (卤0.1 卤0.1 nmol/mg propron SOD activity: (135.2 卤26.9) Umg pro vs (104.3 卤33.1) Umg proproH-Px activity: (21.7 卤4.3) 渭 pro vs (15.6 卤4.0 Umg / P 0.05, but no statistical difference was found between SD1 group and SD1 group. The pathological changes of testis in SD1 group were not obvious. In SD2 group, the tubule lumen of testicular seminiferous tubules became smaller, the peripheral interstitium increased, and the interstitial hyperemia and edema were obvious. Conclusion: long term deep slow wave sleep deprivation can damage the testis and induce oxidative stress.
【作者单位】：天津医科大学代谢病医院内分泌研究所卫生部激素与发育重点实验室天津市代谢性疾病重点实验室;天津医科大学基础医学院;天津医科大学第二临床医学院;
【基金】：天津医科大学大学生学术研究资助计划(TMUUROP2015-08,TMUUROP2016-04);天津医科大学基础医学院大学生科研基金~~
【分类号】：R740

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