全自动免疫组化仪在标准化染色质量控制中的应用

发布时间：2018-07-01 16:00

本文选题：临床病理诊断 + 免疫组织化学　；参考：《中国医学装备》2017年03期

【摘要】：目的:探讨全自动免疫组化仪在标准化染色质量控制中的应用。方法:选择广谱细胞角蛋白(CKpan)、雌激素受体(ER)、突触素(Syn)、上皮生长因子受体(EGFR)及C-erbB-2蛋白(HER2)等34种抗体(一抗),使用统一的第二抗体(二抗),分别采用手工操作和全自动免疫组化仪两种方法对340张病理切片进行染色,统计比较两种方法的染色优良率,同时回顾性分析室间质量控制染色评价结果。结果:两种方法染色质量结果比较:1手工操作的170张病理切片中染色质量优等101张(占59.4%),良49张(占28.8%),差20张(占11.8%),优良率为88.2%;2全自动免疫组化仪染色的170张病理切片中染色质量优等140张(占82.3%),良28张(占16.5%),差2张(占1.2%),优良率为98.8%;两种方法优良率比较差异显著有统计学意义(x~2=15.746,P0.05);室间质量控制平均判读符合率为97.9%,染色符合率为96.0%,整体符合率为93.9%。结论:全自动免疫组化仪染色标准化程度高,染色均匀,定位准确,背景干净,无边缘效应,重复性好,能保证染色结果的准确与稳定,为临床病理诊断、判断预后和指导用药提供科学、准确及可靠的依据。
[Abstract]:Objective: to explore the application of automatic immunohistochemical instrument in the quality control of standardized staining. Methods: Thirty-four kinds of antibodies (first antibody), including broad-spectrum cytokeratin (CKpan), estrogen receptor (ER), synaptophysin (Syn), epithelial growth factor receptor (EGFR) and C-erbB-2 protein (HER2) were selected, and the second antibody (second antibody) was used. 340 pathological sections were stained by two methods. The dyeing rate of two methods was statistically compared and the results of indoor quality control staining were analyzed retrospectively. Results: the results of staining quality of the two methods were compared with those of 170 pathological sections manually operated by VW 1: 101 (59.4%) with excellent staining quality, 49 (28.8%) with good staining quality, 20 (11.8%) with poor staining quality. The excellent and good rate was 88.2% (11.8%). The excellent and good rate was 88.2%. Among the sections, 140 (82.3%) were excellent in dyeing quality, 28 (16.5%) were good, 2 (1.2%) were poor, and the excellent and good rate was 98.8%. The combined rate was 96.0 and the overall coincidence rate was 93.9. Conclusion: the automatic immunohistochemical staining instrument has the advantages of high standardization, uniform staining, accurate location, clean background, no edge effect and good reproducibility, which can ensure the accuracy and stability of the staining results and can be used as a clinicopathological diagnosis. Judgment of prognosis and guidance of medication provide scientific, accurate and reliable basis.
【作者单位】：广州军区广州总医院病理科;
【分类号】：R446.6

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