沙门菌基质辅助激光解吸电离飞行时间质谱方法研究

发布时间：2018-07-02 19:52

本文选题：基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS) + 沙门菌　；参考：《中国人兽共患病学报》2017年08期

【摘要】：目的优化基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)法对沙门菌鉴定的条件,研究不同前处理方法对MALDI靶板污染情况,建立安全高效的检测沙门菌的方法。方法应用MALDI-TOF-MS法对4株沙门菌标准菌株进行不同培养条件、样品前处理方式检验,确立优化的鉴定程序,对不同处理方式下MALDI靶板进行检测,采用优化的前处理方法对33株腹泻病人粪便标本中分离的沙门菌进行数据采集和图谱比对,所得鉴定结果与常规血清学分型结果比较。结果不同前处理方法、不同的培养基上沙门菌标准菌株均准确鉴定为沙门菌。33株沙门菌用MALDI-TOF-MS方法全部准确鉴定为沙门菌属,19株沙门菌在种的鉴定水平上与血清分型结果完全一致,14株沙门菌在种的鉴定水平上与血清分型结果不一致。不同前处理方法的MALDI靶板上均未发现细菌生长。结论 MALDI-TOF-MS方法能快速、简便、安全、准确鉴定属水平沙门菌,是一种有效的沙门菌检测鉴定的手段。
[Abstract]:Objective to optimize the conditions for identification of Salmonella by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), to study the contamination of MALDI target plate by different pretreatment methods and to establish a safe and efficient method for the detection of Salmonella. Methods by using MALDI-TOF-MS method, four standard Salmonella strains were tested under different culture conditions, sample pre-treatment methods, and optimized identification procedures were established, and MALDI target plates were detected under different treatments. The data of 33 strains of Salmonella isolated from fecal specimens of diarrhea patients were collected and compared with the results of routine serological typing. Results different pretreatment methods, The standard Salmonella strains on different culture medium were all identified as Salmonella. 33 Salmonella strains. All of them were identified by MALDI-TOF-MS. All of them were identified as Salmonella. 19 strains of Salmonella were identified at the level of species identification and the results of serological typing were completely consistent with the results of serological typing of 14 strains of Salmonella. The identification level of Bacillus spp. Is inconsistent with the results of serotyping. No bacteria growth was found on the MALDI target plate of different pretreatment methods. Conclusion MALDI-TOF-MS is a rapid, simple, safe and accurate method for the detection and identification of salmonella.
【作者单位】：北京市朝阳区疾病预防控制中心;北京市疾病预防控制中心北京市预防医学研究中心;
【基金】：北京市开放基金(No.2015BZ0063-03)~~
【分类号】：R446.5;R516.3

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