广东省结核分枝杆菌MIRU和RD105缺失基因分型研究
发布时间：2018-07-17 20:09
【摘要】：目的了解广东省结核分枝杆菌(MTB)随时间、地区的动态流行分布特点。方法应用24位点MTB散在分布重复单位(MIRU)分析广东省2013年455例MTB,筛选适合广东省分型的最佳MIRU组合,然后应用该最佳MIRU组合分析广东省2009年的173例、2013年的455例及2015年的225例MTB,此外,结合RD105缺失基因法鉴定这些菌株中的北京家族菌株,比例法进行药物敏感性试验(DST),聚类分析采用Bio Numerics(7.6)。结果 2015年北京家族菌株比率(70.2%)高于2009年(59.5%)和2013年(59.3%),其中,中部地区比率最高(71.3%),东部地区最低(55.0%)。MIRU-24位点的多态性存在差异,MIRU-12(HGI=0.999 1)能达到MIRU-15(HGI=0.999 2)相近的分辨率,MIRU-8分辨率较好(HGI=0.998 5)。853例MTB的系统发生树(MST)主要包括1个北京家族和2个非北京家族复合群,可分为Ⅰ、Ⅱ两群,广东省MTB的主要流行群是Ⅱ群,Ⅰ群中2009、2013年菌株比率高于2015年,东部地区菌株比率高(43.069%),中部地区低(27.206%),Ⅱ群则与之相反,北京家族菌株的MST主要包括1个复合群,包含407个独特型和46个簇。非北京家族菌株的MST主要包括2个复合群,包含309个独特型和6个簇。另外,广东省不同年份多药耐药(MDR)(χ~2=2.176,P=0.337)和广泛耐药(XDR)(χ~2=1.468,P=0.480)耐药率差异无统计学意义(P0.05)。结论北京家族菌株是广东省的主要流行株,且随时间存在上升趋势,中部地区比率最高,该省MDR和XDR耐药趋势相对稳定,MIRU分型用于广东省MTB的分子流行病学研究可取得满意结果。
[Abstract]:Objective to investigate the dynamic distribution of Mycobacterium tuberculosis (MTB) in Guangdong Province over time and region. Methods the 24 locus MTB scattered distributed repeat unit (MIRU) was used to analyze 455 MTBs in Guangdong province in 2013. Then the best MIRU combination was used to analyze 173 cases of MTBs in Guangdong Province in 2009, 455 cases in 2013 and 225 cases in 2015. In addition, the strains of Beijing family were identified by using RD105 deletion gene method. Drug sensitivity test (DST) was performed by proportional method and Bio Numerics (7.6) was used for cluster analysis. Results the percentage of strains in Beijing in 2015 (70.2%) was higher than that in 2009 (59.5%) and 2013 (59.3%). The central region has the highest ratio (71.3%), and the eastern region has the lowest (55.0%) .MIRU-24 polymorphism. MIRU-12 (HGIJ 0.999 1) can reach the similar resolution of MIRU-15 (HGIJ 0.999 2). The resolution of MIRU-8 is better (HGI0.998 5) .853 cases of MTB phylogenetic tree (MST) mainly include one Beijing family and two non-MTB phylogenetic trees (MST). Beijing family complex, It can be divided into two groups: group 鈪，
本文编号：2130800
[Abstract]:Objective to investigate the dynamic distribution of Mycobacterium tuberculosis (MTB) in Guangdong Province over time and region. Methods the 24 locus MTB scattered distributed repeat unit (MIRU) was used to analyze 455 MTBs in Guangdong province in 2013. Then the best MIRU combination was used to analyze 173 cases of MTBs in Guangdong Province in 2009, 455 cases in 2013 and 225 cases in 2015. In addition, the strains of Beijing family were identified by using RD105 deletion gene method. Drug sensitivity test (DST) was performed by proportional method and Bio Numerics (7.6) was used for cluster analysis. Results the percentage of strains in Beijing in 2015 (70.2%) was higher than that in 2009 (59.5%) and 2013 (59.3%). The central region has the highest ratio (71.3%), and the eastern region has the lowest (55.0%) .MIRU-24 polymorphism. MIRU-12 (HGIJ 0.999 1) can reach the similar resolution of MIRU-15 (HGIJ 0.999 2). The resolution of MIRU-8 is better (HGI0.998 5) .853 cases of MTB phylogenetic tree (MST) mainly include one Beijing family and two non-MTB phylogenetic trees (MST). Beijing family complex, It can be divided into two groups: group 鈪，
本文编号：2130800
本文链接：https://www.wllwen.com/linchuangyixuelunwen/2130800.html
最近更新
教材专著
