荧光免疫试条读数仪量程调节方法研究

发布时间：2018-08-13 17:30

【摘要】：随着荧光免疫层析技术朝着高灵敏度、宽检测范围的方向发展,对配套荧光免疫试条读数仪的检测要求也随之提高。通过研究荧光产生机理,建立调节量程的理论模型并提出一种提高荧光免疫试条读数仪检测上限和检测灵敏度的方法。设计数控恒流源调整光源的激发光强,实现对试条荧光信号的控制,从而调节荧光免疫试条读数仪检测量程。实验表明,该方法使荧光免疫试条读数仪具备更宽的试条浓度检测范围,有效提高其检测上限与检测灵敏度。实验以C-反应蛋白(CRP)作为待测物质,结果表明荧光免疫试条读数仪检测上限较调整前提高4倍,检测灵敏度提高1倍,在2~0~2~(10)μg/m L浓度范围内检测结果的线性拟合优度R~20.99,与ESE的Quant分析仪在相同检测范围内的准确度一致性较好。
[Abstract]:With the development of fluorescent immunochromatographic technology towards high sensitivity and wide detection range, the detection requirements of the matched fluorescent immunoassay readout are also increased. By studying the mechanism of fluorescence generation, a theoretical model of adjusting range is established and a method to improve the detection upper limit and detection sensitivity of fluorescence immunoassay is proposed. A numerical controlled constant current source is designed to adjust the excitation intensity of the light source, to control the fluorescence signal of the strip, and thus to adjust the measuring range of the fluorescent immunoassay reading meter. The experimental results show that this method can make the fluorescent immunoassay have a wider range of test strip concentration and effectively improve the detection limit and sensitivity. C- reactive protein (CRP) was used as the material to be tested. The results showed that the upper limit of fluorescence immunoassay was 4 times higher than that before adjustment, and the detection sensitivity was 1 times higher than that before adjustment. In the range of 2 ~ (10) 渭 g 路mL ~ (10) 渭 g 路mL ~ (-1) ~ (10) 渭 g ~ (10) 渭 g / mL, the linear goodness of fit (R ~ (20. 99) of the detection results is better than that of the ESE Quant analyzer in the same range of accuracy.
【作者单位】：福州大学物理与信息工程学院;福州大学福建省医疗器械和医药技术重点实验室;澳门大学模拟与混合信号超大规模集成电路国家重点实验室;
【基金】：科技部港澳台科技合作项目(2012DFM30040) 福建省科技重大专项(2014YZ0001)
【分类号】：R446.6

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