具有光动力治疗效果的超分子聚合物比率式纳米氧气传感器
发布时间：2018-08-26 18:39
【摘要】：低氧是许多疾病产生的信号,例如癌症、心血管疾病、中风、阿兹海默病和帕金森病等,所以及时检测人体细胞和组织中氧气对癌症的诊断至关重要。基于磷光猝灭的比率式光学氧气传感器灵敏度高、选择性好、可逆性好以及检测时无需消耗氧气受到广大科研工作者的关注。光动力疗法(PDT)技术相比化学疗法具有副作用小、抗药性小和低毒性等优点,对癌症的治疗极具潜力。因此,发展具有光动力治疗效果的多功能氧气传感器具有重要的意义。超分子聚合物由小分子单体通过可逆的非共价键作用相连接共聚而成,其组份和功能可以通过单体结构的改变来调节,为构筑比率式光学氧气传感器提供了基础。本论文开展了以下三部分的研究:1、合成了具有双四重氢键的磷光化合物Por(Pt)-bisUPy和荧光化合物DPA-bisUPy,它们的结构通过核磁共振氢谱、核磁共振碳谱及高分辨质谱进行了表征,这两种化合物的光物理性质能较好满足构筑比率式氧气传感器的要求。2、通过核磁氢谱和粘度测试证明了化合物DPA-bisUPy和Por(Pt)-bisUPy在氯仿中可以形成超分子聚合物链;通过微乳液法制备了超分子聚合物纳米球NP1、NP2、NP3,通过SEM和TEM表征,表明这三种超分子聚合物纳米球具有规整的形貌与尺寸,粒径大约为60 nm左右;能量给体DPA-bisUPy与能量受体Por(Pt)-bisUPy之间存在高效的能量传递,进而显著提高氧气传感器的磷光量子效率;氧气猝灭研究结果表明该传感器对氧气具有高的灵敏度,达到了生物学测试的要求,它不仅是比率式还是比色式光学氧气传感器。3、超分子聚合物比率式纳米氧气传感器具有良好的光稳定性以及较好的生物相容性,可用于光动力治疗和细胞成像。
[Abstract]:Hypoxia is the signal of many diseases, such as cancer, cardiovascular disease, stroke, Alzheimer's disease and Parkinson's disease, so the timely detection of oxygen in human cells and tissues is essential for the diagnosis of cancer. The ratio optical oxygen sensor based on phosphorescence quenching is of high sensitivity, good selectivity, good reversibility and no oxygen consumption in detection. Compared with chemotherapy, photodynamic therapy (PDT) has the advantages of less side effect, less drug resistance and less toxicity, so it has great potential in the treatment of cancer. Therefore, it is of great significance to develop a multifunctional oxygen sensor with photodynamic effect. Supramolecular polymers are composed of small molecular monomers linked and copolymerized by reversible noncovalent bonding. Their composition and function can be adjusted by the change of monomer structure, which provides the basis for the construction of proportional optical oxygen sensors. In this thesis, we have studied the following three parts: 1, and synthesized phosphorescence compound Por (Pt) bisUPy with double quadruple hydrogen bond and fluorescent compound DPA-bisUPy,. Their structures were characterized by NMR, NMR carbon and high resolution mass spectrometry. The photophysical properties of these two compounds can meet the requirements of constructing a ratio oxygen sensor. The results of NMR and viscosity measurements show that the compounds DPA-bisUPy and Por (Pt) bisUPy can form supramolecular polymer chains in chloroform. The supramolecular polymer nanospheres (NP1,NP2,NP3,) were prepared by microemulsion method and characterized by SEM and TEM. The results show that the three supramolecular polymer nanospheres have regular morphology and size, and the particle size is about 60 nm. There is a high energy transfer between the energy donor DPA-bisUPy and the energy receptor Por (Pt) bisUPy, which can significantly improve the phosphorescence quantum efficiency of the oxygen sensor, and the oxygen quenching results show that the sensor has a high sensitivity to oxygen. It is not only a ratio or colorimetric optical oxygen sensor .3. the supramolecular polymer ratio nano-oxygen sensor has good photostability and good biocompatibility. It can be used in photodynamic therapy and cell imaging.
【学位授予单位】：中北大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R454;TP212
本文编号：2205808
[Abstract]:Hypoxia is the signal of many diseases, such as cancer, cardiovascular disease, stroke, Alzheimer's disease and Parkinson's disease, so the timely detection of oxygen in human cells and tissues is essential for the diagnosis of cancer. The ratio optical oxygen sensor based on phosphorescence quenching is of high sensitivity, good selectivity, good reversibility and no oxygen consumption in detection. Compared with chemotherapy, photodynamic therapy (PDT) has the advantages of less side effect, less drug resistance and less toxicity, so it has great potential in the treatment of cancer. Therefore, it is of great significance to develop a multifunctional oxygen sensor with photodynamic effect. Supramolecular polymers are composed of small molecular monomers linked and copolymerized by reversible noncovalent bonding. Their composition and function can be adjusted by the change of monomer structure, which provides the basis for the construction of proportional optical oxygen sensors. In this thesis, we have studied the following three parts: 1, and synthesized phosphorescence compound Por (Pt) bisUPy with double quadruple hydrogen bond and fluorescent compound DPA-bisUPy,. Their structures were characterized by NMR, NMR carbon and high resolution mass spectrometry. The photophysical properties of these two compounds can meet the requirements of constructing a ratio oxygen sensor. The results of NMR and viscosity measurements show that the compounds DPA-bisUPy and Por (Pt) bisUPy can form supramolecular polymer chains in chloroform. The supramolecular polymer nanospheres (NP1,NP2,NP3,) were prepared by microemulsion method and characterized by SEM and TEM. The results show that the three supramolecular polymer nanospheres have regular morphology and size, and the particle size is about 60 nm. There is a high energy transfer between the energy donor DPA-bisUPy and the energy receptor Por (Pt) bisUPy, which can significantly improve the phosphorescence quantum efficiency of the oxygen sensor, and the oxygen quenching results show that the sensor has a high sensitivity to oxygen. It is not only a ratio or colorimetric optical oxygen sensor .3. the supramolecular polymer ratio nano-oxygen sensor has good photostability and good biocompatibility. It can be used in photodynamic therapy and cell imaging.
【学位授予单位】：中北大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R454;TP212
【参考文献】
相关期刊论文 前3条
1 彭洪尚;申睿颖;王小卉;;面向生物医学应用的光学氧气纳米传感器[J];发光学报;2016年10期
2 王瑞芳;高建峰;陈鹏忠;陈玉哲;杨清正;;基于磷光猝灭的比率式光学氧气探针[J];科学通报;2015年04期
3 李勃天;唐黎明;邱义鹏;王宇;;具有四重氢键识别基元超支化聚合物的特殊流变行为[J];高分子学报;2009年06期
，本文编号：2205808
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