氯胺酮鞘内给药对神经病理性痛大鼠脊髓背角小胶质细胞活化、TNF-α和IL-1β产生的影响

发布时间：2018-09-07 14:54

【摘要】：目的:探究鞘内注射氯胺酮(ketamine,KTM)对坐骨神经结扎(CCI)神经病理性疼痛大鼠模型行为、脊髓背角肿瘤坏死因子-α(TNF-α)和白介素1β(IL-1β)以及小胶质细胞标记蛋白OX42表达的影响。方法:(1)建立CCI模型大鼠,鞘内注射KTM或MI(米诺四环素)进行干预,测定大鼠机械缩足阈值(MWT)和热缩足潜伏期(TWL)。(2)运用ELISA法检测TNF-α和IL-1β表达水平。(3)使用Western Blot法检测OX42的表达情况。结果:(1)KTM组和MI组大鼠MWT和TWL值较CCI组显著升高;KTM组大鼠MWT和TWL值高于CCI组(P0.05)。(2)KTM组和MI组TNF-α和IL-1β表达水平低于CCI组(P0.05);其中KTM组TNF-α和IL-1β表达水平显著高于MI组(P0.05)。(3)KTM组和MI组OX42表达水平显著低于CCI组;KTM组OX42表达水平高于MI组(P0.05)。结论:鞘内KTM可以抑制脊髓背角小胶质细胞激活,减少TNF-α和IL-1β表达,显著改善坐骨神经结扎引起的神经病理性疼痛。
[Abstract]:Aim: to investigate the effects of intrathecal injection of ketamine (ketamine,KTM) on the expression of tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 尾 (IL-1 尾) and microglial marker protein (OX42) in the spinal dorsal horn of rats with (CCI) neuropathic pain following sciatic nerve ligation. Methods: (1) CCI model rats were established. KTM or MI (minocycline) were injected intrathecally. The mechanical foot contraction threshold (MWT) and thermal contraction latency (TWL). (_ 2) were measured. The expression of TNF- 伪 and IL-1 尾 was detected by ELISA method. (3) OX42 expression was detected by Western Blot method. Results: (1) the expression of TNF- 伪 and IL-1 尾 in KTM group and MI group were significantly higher than those in CCI group (P0.05). (2), and the expression levels of TNF- 伪 and IL-1 尾 in KTM group were significantly higher than those in MI group (). (3) KTM group and MI group (P0.05). The expression of OX42 in KTM group was significantly lower than that in MI group (P0.05). Conclusion: intrathecal KTM can inhibit the activation of spinal dorsal horn microglia, decrease the expression of TNF- 伪 and IL-1 尾, and significantly improve the neuropathic pain caused by sciatic nerve ligation.
【作者单位】：遵义医学院附属医院重症医学科;遵义医学院附属医院疼痛科;贵州省麻醉与器官保护重点实验室;
【分类号】：R402

【相似文献】