多重耐药鲍曼不动杆菌的临床流行特征

发布时间：2018-10-08 19:10

【摘要】：目的对西安交通大学第一附属医院收集的多重耐药鲍曼不动杆菌进行菌种确认,并检测其临床感染分布特点、耐药性及分子分型特征。方法收集经VITEK-2鉴定的多重耐药鲍曼不动杆菌47株,利用特异性引物sp2F、sp4F和sp4R通过PCR扩增的方法复检菌株;使用VITEK-2检测鲍曼不动杆菌的药物敏感性;通过多位点序列分型(MLST)方法分析临床菌株的同源性。结果经过特异性PCR扩增法确认,47株临床样本中有46株为鲍曼不动杆菌,它们大多分离自痰标本,主要收集于重症监护室,呈多重耐药性。MLST分析发现ST195、ST218、ST368及ST208共4种ST型,其中ST368与ST208亲缘关系最近。结论特异性PCR扩增法可快速、准确地鉴定鲍曼不动杆菌。本院的多重耐药鲍曼不动杆菌主要分布在重症监护室,仅对替加环素等少数抗生素有较高敏感性,存在同源性差异。
[Abstract]:Objective to identify the multidrug resistant Acinetobacter baumannii collected from the first affiliated Hospital of Xi'an Jiaotong University, and to detect its clinical infection distribution, drug resistance and molecular typing. Methods A total of 47 strains of Acinetobacter baumannii identified by VITEK-2 were collected and retested by PCR amplification with specific primer sp2F,sp4F and sp4R, and the drug sensitivity of Acinetobacter baumannii was detected by VITEK-2. The homology of clinical strains was analyzed by multiple locus sequence typing (MLST). Results 46 of 47 clinical strains were identified as Acinetobacter baumannii by specific PCR amplification. Most of them were isolated from sputum and collected mainly in intensive care unit. The results showed that there were four ST types of ST195,ST218,ST368 and ST208 by multidrug resistance. The relationship between ST368 and ST208 was the most recent. Conclusion the specific PCR amplification method can be used to identify Acinetobacter baumannii quickly and accurately. Acinetobacter baumannii which is multidrug resistant in our hospital is mainly distributed in intensive care unit and has high sensitivity to a few antibiotics such as tegicycline.
【作者单位】：西安交通大学医学部基础医学院病原生物学与免疫学系;环境与疾病相关基因教育部重点实验室;西安交通大学第一附属医院检验科;陕西省人民医院心内二科;西安交通大学第一附属医院肝胆外科;
【基金】：陕西省自然科学基础研究计划青年人才项目(No.2016JQ8027,2016JQ8029) 中央高校基本科研业务费专项资金(No.XJJ2017140)~~
【分类号】：R446.5

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