改良采集模式在外周血干细胞采集中的应用

发布时间：2018-11-02 19:37

【摘要】：目的研究费森尤斯血细胞分离机的改良采集模式在外周血干细胞采集中的应用效果。方法 2013年1月-2016年12月,将火箭军总医院135名行外周血造血干细胞采集术的健康供者或自体采集患者分成2组,使用费森尤斯血细胞分离机,对照组采用机器默认模式进行采集;实验组改良采集模式,通过手动调节模式进行采集。将2组采集后的干细胞质量进行比较,同时比较2组采集模式对供者的外周血液指标的影响。结果 2种采集模式对供/患者的Hb、RBC、Hct和WBC结果的影响无统计学意义(P0.05),实验组的Plt降幅小于对照组(P0.05);实验组的产品体积少于对照组,细胞浓度高于对照组(P0.01);但实验组采集所耗时间高于对照组(P0.05)。结论改良外周血干细胞的采集模式,可最大限度地减少供/患者血小板的损失,提高了PBSC终产品的质量。手动模式在短时间内大容量采集干细胞上具有优势。2种模式各有优缺点,应根据供/患者的具体情况选择不同的采集模式,以达到最佳的采集效果。
[Abstract]:Objective to study the application effect of improved collection model of Fessenius blood cell separator in peripheral blood stem cell collection. Methods from January 2013 to December 2016, 135 healthy donors or patients with autologous collection of peripheral blood stem cells were divided into two groups. In the control group, the machine default mode was used to collect the data. The experimental group improved the acquisition mode and collected by manual adjustment mode. The quality of stem cells collected from the two groups was compared and the effects of the two collection models on the peripheral blood fluid indexes of the donors were compared at the same time. Results the results of Hb,RBC,Hct and WBC of donor / patient were not significantly affected by the two sampling models (P0.05), but the decrease of Plt in the experimental group was lower than that in the control group (P0.05). The product volume of the experimental group was less than that of the control group, and the cell concentration was higher than that of the control group (P0.01); Conclusion the improved collection model of peripheral blood stem cells can minimize the platelet loss of donor / patient and improve the quality of PBSC final products. The manual mode has advantages in collecting stem cells in a short period of time, and the two models have their own advantages and disadvantages. Different acquisition modes should be selected according to the specific conditions of the donor / patient to achieve the best collection effect.
【作者单位】：北京市红十字血液中心;火箭军总医院;
【基金】：国家自然科学基金项目(31600681,81400274)
【分类号】：R457.7

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