冻干红细胞残余水的测定及其分布研究
发布时间：2018-11-06 14:46
【摘要】：目的建立并比较冻干红细胞残余水含量测定方法,探讨冻干红细胞残余水的分布情况。方法一次性冻干等量(500μL)的含保护剂(主要成分12%甘油)的红细胞、无保护剂的红细胞,含保护剂(同前)的血影细胞、无保护剂的血影细胞及保护剂,用热重分析法及Karl-Fisher滴定法结合卡式炉法测定其残余水含量,同时用含水量固定的标准品(水分含量4.9%-5.2%)做检验,每组样品重复测量6次;分析各组之间的差异,比较冻干血影细胞及冻干红细胞残余水含量。结果热重分析法测定残余水含量(%):冻干红细胞、无保护剂冻干红细胞、冻干血影细胞、无保护剂冻干血影细胞、单纯冻干保护剂、标准品分别为19.01±2.18、4.60±0.78、18.95±1.89、4.87±1.01、16.12±1.04、5.28±0.16;冻干保护剂及含保护剂冻干红细胞的热重(TG)曲线走势接近,无保护剂冻干红细胞与前二者曲线走势差异明显;Karl-Fisher法测定残余水含量(%):冻干红细胞、无保护剂冻干红细胞、冻干血影细胞、无保护剂冻干血影细胞、单纯冻干保护剂、标准品分别为3.21±0.23、1.22±0.09、3.16±0.26、1.25±0.07、2.63±0.41、5.14±0.13。结论热重分析法测定含保护剂的冻干品残余水含量时的结果偏高,Karl-Fisher滴定法结合卡式炉法能够准确反映冻干红细胞的残余水含量;初步判断冻干红细胞残余水主要分布于细胞膜。
[Abstract]:Objective to establish and compare the method for the determination of residual water in freeze-dried red blood cells and to study the distribution of residual water in freeze-dried red blood cells. Methods RBC containing protective agent (12% glycerol), erythrocyte without protective agent, blood shadow cell containing protective agent (same as before), blood shadow cell without protective agent and protective agent were freeze-dried at one time (500 渭 L). The residual water content was determined by thermogravimetric analysis and Karl-Fisher titration combined with calcareous furnace method. At the same time, the content of residual water was tested with the standard sample with water content fixed (4.9- 5.2%), and the samples were measured six times repeatedly in each group. The difference between each group was analyzed and the residual water content of freeze-dried blood shadow cells and freeze-dried red blood cells was compared. Results the residual water content (%) was determined by thermogravimetric analysis: freeze-dried red blood cells, freeze-dried erythrocytes, freeze-dried blood shadow cells, freeze-dried blood shadow cells without protective agents, simple freeze-dried protective agents. The standard samples were 19.01 卤2.180.60 卤0.78U 18.95 卤1.89C 4.87 卤1.01C 16.12 卤1.04U 5.28 卤0.16, respectively. The trend of thermogravimetric (TG) curve of freeze-dried erythrocytes was close to that of freeze-dried erythrocytes, but the trend of freeze-dried erythrocytes without protective agents was obviously different from that of the former. The content of residual water (%) was determined by Karl-Fisher: freeze-dried red blood cells, freeze-dried erythrocytes, freeze-dried blood shadow cells, freeze-dried blood shadow cells without protective agents, simple freeze-dried protectants. The standard samples were 3.21 卤0.23C 1.22 卤0.09U 3.16 卤0.26C 1.25 卤0.072.63 卤0.41g 5.14 卤0.13, respectively. Conclusion the results of thermogravimetric analysis for the determination of residual water in freeze-dried products containing protective agents are high. Karl-Fisher titration combined with calcareous furnace method can accurately reflect the residual water content of freeze-dried red blood cells. It was preliminarily estimated that the residual water of freeze-dried red blood cells was mainly distributed in the cell membrane.
【作者单位】： 安徽医科大学公共卫生学院;中国医学科学院北京协和医学院输血研究所;
【基金】：国家自然科学基金面上项目(81370669)
【分类号】：R457.1
本文编号：2314590
[Abstract]:Objective to establish and compare the method for the determination of residual water in freeze-dried red blood cells and to study the distribution of residual water in freeze-dried red blood cells. Methods RBC containing protective agent (12% glycerol), erythrocyte without protective agent, blood shadow cell containing protective agent (same as before), blood shadow cell without protective agent and protective agent were freeze-dried at one time (500 渭 L). The residual water content was determined by thermogravimetric analysis and Karl-Fisher titration combined with calcareous furnace method. At the same time, the content of residual water was tested with the standard sample with water content fixed (4.9- 5.2%), and the samples were measured six times repeatedly in each group. The difference between each group was analyzed and the residual water content of freeze-dried blood shadow cells and freeze-dried red blood cells was compared. Results the residual water content (%) was determined by thermogravimetric analysis: freeze-dried red blood cells, freeze-dried erythrocytes, freeze-dried blood shadow cells, freeze-dried blood shadow cells without protective agents, simple freeze-dried protective agents. The standard samples were 19.01 卤2.180.60 卤0.78U 18.95 卤1.89C 4.87 卤1.01C 16.12 卤1.04U 5.28 卤0.16, respectively. The trend of thermogravimetric (TG) curve of freeze-dried erythrocytes was close to that of freeze-dried erythrocytes, but the trend of freeze-dried erythrocytes without protective agents was obviously different from that of the former. The content of residual water (%) was determined by Karl-Fisher: freeze-dried red blood cells, freeze-dried erythrocytes, freeze-dried blood shadow cells, freeze-dried blood shadow cells without protective agents, simple freeze-dried protectants. The standard samples were 3.21 卤0.23C 1.22 卤0.09U 3.16 卤0.26C 1.25 卤0.072.63 卤0.41g 5.14 卤0.13, respectively. Conclusion the results of thermogravimetric analysis for the determination of residual water in freeze-dried products containing protective agents are high. Karl-Fisher titration combined with calcareous furnace method can accurately reflect the residual water content of freeze-dried red blood cells. It was preliminarily estimated that the residual water of freeze-dried red blood cells was mainly distributed in the cell membrane.
【作者单位】： 安徽医科大学公共卫生学院;中国医学科学院北京协和医学院输血研究所;
【基金】：国家自然科学基金面上项目(81370669)
【分类号】：R457.1
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