6周有氧运动对高脂饲养大鼠性激素及组织芳香化酶表达量的影响

发布时间：2018-11-11 09:19

【摘要】：目的:观察高脂膳食对雄性大鼠性激素、性腺和性腺外组织中芳香化酶表达量的影响及自由转轮运动的干预效果,探讨有氧运动影响高脂膳食大鼠性激素水平的可能机制。方法:40只3周龄雄性SD大鼠随机分为4组:普通膳食对照组(C组)、普通膳食运动组(E组)、高脂膳食组(D组)、高脂膳食运动组(Er D组)。E、ErD两组进行自由转轮运动,每天2h,每周训练5天。6周后腹主动脉取血,制备血清,并取睾丸、肝脏、股四头肌和肾脏,检测总睾酮(T)和雌二醇(E_2)含量,并检测组织中芳香化酶(P450 arom)的表达情况。结果:(1)与C组相比,D组大鼠血清T显著降低(P0.05),E_2显著升高(P0.05);与D组相比,ErD组大鼠T水平升高,但是无显著性,E_2水平明显下降(P0.05)。(2)与C组相比,D组大鼠睾丸和股四头肌中P450 arom表达量显著升高(P0.05),肝脏和肾脏中非常显著性升高(P0.01);与D组相比,ErD组大鼠睾丸和肾脏中P450 arom表达量显著降低(P0.01,P0.05),肝脏和股四头肌中也出现降低,但差异不明显(P0.05)。结论:高脂膳食可引起雄性大鼠睾酮水平降低和雌二醇水平增高。6周自由转轮运动一定程度上改善了高脂膳食诱发的性激素水平异常现象,其作用可能与降低性腺(睾丸)及性腺外组织芳香化酶的表达有关。
[Abstract]:Objective: to observe the effect of high fat diet on the expression of aromatase in sex hormone, gonad and extragonadal tissues of male rats, and to explore the possible mechanism of aerobic exercise on sex hormone level in rats with high fat diet. Methods: forty 3-week-old male SD rats were randomly divided into 4 groups: normal diet control group (C group), general diet exercise group (E group), high fat diet group (D group), high fat diet exercise group (Er D group). E, The ErD group was treated with free wheel exercise for 2 hours a day for 5 days a week. After 6 weeks of training, blood was taken from the abdominal aorta to prepare serum, testis, liver, quadriceps femoris and kidney were taken, and total testosterone (T) and estradiol (E2) were measured. The expression of aromatase (P450 arom) was detected. Results: (1) compared with group C, serum T in group D was significantly lower than that in group C (P0.05) and E2 was significantly increased (P0.05). Compared with group D, the expression of P450 arom in testis and quadriceps femoris in group D was significantly higher than that in group D (P0.05), but there was no significant difference between group D and group D (P 0.05), but there was no significant difference between group D and group C in the expression of P450 arom in testis and quadriceps femoris (P0.05). There was a significant increase in liver and kidney (P0.01). Compared with group D, the expression of P450 arom in testis and kidney of ErD group was significantly lower than that in group D (P0.05), and the expression of P450 arom in liver and quadriceps femoris was also decreased, but the difference was not significant (P0.05). Conclusion: high fat diet can decrease testosterone level and increase estradiol level in male rats. 6 weeks free wheel exercise can improve the abnormal sex hormone level induced by high fat diet to some extent. Its effect may be related to the reduction of aromatase expression in gonads (testis) and extragonadal tissues.
【作者单位】：北京体育大学运动人体科学学院;国家体育总局运动医学研究所;
【基金】：国家自然科学基金青年项目(30800541)
【分类号】：R493

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