pLXSN-Tum-5致人脐静脉内皮细胞凋亡作用及机制

发布时间：2018-11-17 08:38

【摘要】：目的探讨pLXSN-Tum-5病毒颗粒对人脐静脉内皮细胞(HUVEC)的生长抑制和诱导凋亡作用及机制。方法采用不同浓度的pLXSN-Tum-5病毒颗粒作用HUVEC,利用噻唑蓝法和Hoechst-33342染色法检测细胞生长及其形态变化;RT-PCR和Western blotting方法检测细胞中Bax、Bcl-2、caspase-3 mRNA和蛋白表达变化。结果与对照组(pLXSN)比较,pLXSN-Tum-5病毒颗粒转染组HUVEC的存活率明显降低,呈剂量效应关系;细胞内可见凋亡小体;对照组HUVEC内Bcl-2和caspase-3 mRNA和蛋白表达分别为(0.721±0.041)、(0.654±0.034)和(0.956±0.032)、(0.356±0.054);与对照组比较,20μmol/L pLXSN-Tum-5病毒颗粒转染组HUVEC内Bcl-2和caspase-3mRNA和蛋白水平[分别为(1.134±0.0524)、(1.012±0.0641)和(1.612±0.067)、(0.712±0.0647)]明显上调(P0.05)。结论 Tum-5可抑制HUVEC增殖,诱导HUVEC凋亡,其机制可能与上调Bax/Bcl-2和caspase-3表达有关。
[Abstract]:Objective to investigate the effect and mechanism of pLXSN-Tum-5 virus granule on the growth inhibition and apoptosis of human umbilical vein endothelial cells (HUVEC). Methods HUVEC, was treated with different concentrations of pLXSN-Tum-5 virus particles. Cell growth and morphological changes were detected by thiazolyl blue assay and Hoechst-33342 staining. The expression of Bax,Bcl-2,caspase-3 mRNA and protein was detected by RT-PCR and Western blotting. Results compared with the control group, the survival rate of HUVEC in the pLXSN-Tum-5 virus particles transfection group was significantly decreased in a dose-dependent manner, and apoptotic corpuscles were found in the cells. The expression of Bcl-2, caspase-3 mRNA and protein in HUVEC of control group were (0.721 卤0.041), (0.654 卤0.034) and (0.956 卤0.032), (0.356 卤0.054), respectively. Compared with the control group, the levels of Bcl-2, caspase-3mRNA and protein in the HUVEC transfected with 20 渭 mol/L pLXSN-Tum-5 virus particles were (1.134 卤0.0524), (1.012 卤0.0641) and (1.612 卤0.067), respectively. (0.712 卤0.0647) significantly up-regulated (P0.05). Conclusion Tum-5 can inhibit the proliferation of HUVEC and induce HUVEC apoptosis, which may be related to the up-regulation of Bax/Bcl-2 and caspase-3 expression.
【作者单位】：吉林医药学院基础医学院;北华大学基础医学院;
【基金】：吉林省科技厅项目(20130206050YY;20150101128JC)
【分类号】：R450

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