ε-聚赖氨酸抑菌效果及对尿铅测定影响研究

发布时间：2018-11-25 18:54

【摘要】：目的评估ε-聚赖氨酸(ε-PL)对尿样中金黄色葡萄球菌、粪肠球菌、铜绿假单胞菌和大肠杆菌4种常见腐败菌的抑菌效果及其对尿铅测定结果的影响。方法采用肉汤稀释法测定ε-PL对4种常见腐败菌的最小抑菌浓度(MIC);观察终质量浓度为40.000 mg/L的ε-PL对尿样的抑菌效果;用石墨炉原子吸收光谱法测定质量浓度为40.000 mg/L的ε-PL溶液中的铅本底水平和正常健康人群尿样的尿铅水平;比较ε-PL和硝酸对尿样的抑菌效果。结果ε-PL对金黄色葡萄球菌、粪肠球菌、铜绿假单胞菌和大肠杆菌同时具有抑菌效果的MIC为40.000 mg/L。含终质量浓度为40.000 mg/L的ε-PL的尿样在常温下保存24 h~15 d均无细菌生长。质量浓度为40.000 mg/L的ε-PL溶液中铅水平2.0μg/L。分别以质量浓度为40.000 mg/L的ε-PL和体积分数为1.0%硝酸作为防腐剂时,尿样中铅水平的下降率相近,且尿样保存15 d后尿铅水平的下降率均小于10.0%。结论ε-PL可替代硝酸作为尿铅测定样品保存的新型天然防腐剂。
[Abstract]:Objective to evaluate the inhibitory effect of 蔚 -Polylysine (蔚-PL) on four common spoilage bacteria, Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli, and to evaluate the effect of 蔚-PL on the determination of lead in urine. Methods the minimal inhibitory concentration of 蔚-PL against four common spoilage bacteria was determined by broth dilution method. The inhibitory effect of 蔚-PL with final mass concentration of 40.000 mg/L on urine samples was observed by (MIC);. Graphite furnace atomic absorption spectrometry (GFAAS) was used to determine the background level of lead in 蔚-PL solution with mass concentration of 40.000 mg/L and the urine lead level in normal controls, and to compare the bacteriostatic effect of 蔚-PL and nitric acid on urine samples. Results the MIC of 蔚-PL against Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa and Escherichia coli was 40.000 mg/L.. Urine samples containing final concentration of 40.000 mg/L 蔚-PL had no bacterial growth at room temperature for 24 h or 15 d. The lead level in 蔚-PL solution with a mass concentration of 40.000 mg/L was 2.0 渭 g 路L ~ (-1) 路L ~ (-1). When the concentration of 蔚-PL of 40.000 mg/L and the volume fraction of 1.0% nitric acid were used as preservatives, the decrease rate of lead level in urine samples was similar, and the decrease rate of lead level in urine samples was less than 10.0 after 15 days of storage. Conclusion 蔚-PL can replace nitric acid as a new natural preservative for the determination of lead in urine.
【作者单位】：广东省中医院珠海医院;广东省职业病防治院广东省职业病防治重点实验室;广东药科大学公共卫生学院;
【基金】：珠海市卫计局课题(2016J007)
【分类号】：R446.12

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