快速检测沙门氏菌的荧光免疫试纸的研制

发布时间：2018-12-12 17:49

【摘要】：研制一种基于低噪声激发式荧光沙门氏菌免疫层析检测试纸条,用于沙门氏菌快速、高敏、兼顾定性及精准定量的检测。采用低噪声激发式荧光染料作为标记,采用免疫层析技术,制备靶向于沙门氏菌的免疫层析试纸条。检测时,采用专用便携式低噪声激发式荧光扫描仪分别扫描质控线和检测线,以检测线荧光强度检测值实现样本的定性及定量检测,并对免疫层析试纸条各项性能进行评价。低噪声激发式荧光沙门氏菌免疫层析检测试纸条制备成功,免疫层析试纸条性能检测结果显示,该试纸条特异性强、灵敏度高、检测限可达到0.5×10~3 CFU/mL,是一种新型快速高效稳定的检测方法。该免疫层析试纸条可适用于食品及病理样本中沙门氏菌初筛和即时检测。
[Abstract]:A low noise stimulated fluorescent immunochromatographic test strip was developed for rapid, Gao Min, qualitative and quantitative detection of salmonella. The immunochromatographic strip targeting Salmonella was prepared by using low noise excitation fluorescent dye as the label and immunochromatography technique. The quality control line and the detection line were scanned by special portable low-noise excitation fluorescence scanner. The qualitative and quantitative detection of the sample was realized by the fluorescence intensity detection value of the detection line, and the performance of the immunochromatographic strip was evaluated. The low noise stimulated fluorescent Salmonella immunochromatographic test strip was successfully prepared. The results showed that the test strip had strong specificity, high sensitivity and detection limit of 0.5 脳 10 ~ (-3) CFU/mL,. It is a new fast, efficient and stable detection method. The immunochromatographic strip can be used for screening and immediate detection of salmonella in food and pathological samples.
【作者单位】：北京出入境检验检疫局检验检疫技术中心;出入境食品安全检测北京市重点实验室;厦门市产品质量监督检验院;天津出入境检验检疫局工业产品安全技术中心;潍坊高新技术产业开发区人民医院;
【基金】：质检公益性行业科研专项项目(201410049) 国家质检总局科技计划项目(2015IK011)
【分类号】：R446.6

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