黄连、氯己定与抗生素介导的细菌交叉耐药

发布时间：2019-04-04 08:15

【摘要】：目的:研究抗生素、消毒剂(氯己定)和抗菌中药(黄连)能否介导细菌产生交叉耐药。方法:根据国际标准微量肉汤稀释法测定上述药物对金黄色葡萄球菌(Staphylococcus aureus,Sa)的最低抑菌浓度(minimum inhibitory concentration,MIC)。根据抗性结果分析,选择亚致死剂量下的药物浓度连续培养细菌14 d,诱导细菌产生交叉耐药。结果:经环丙沙星(ciprofloxacin,CIP)和庆大霉素(gentamicin,GEN)诱导后细菌没有增加对氯己定(chlorhexidine,CHX)的抗性,但出现了对CHX敏感性提高的现象。分别有1株和4株Sa对黄连水提液(Rhizoma coptidis extract,RCE)和盐酸小檗碱(berberine,BBR)提高了抗性。经四环素(tetracycline,TET)诱导后,对CHX、RCE和BBR产生交叉耐药的Sa菌株分别有3,6,8株。经CHX诱导后,几乎所有菌株都对至少一种抗生素产生了交叉耐药,分别有7株和9株Sa对RCE和BBR抗性提高了4倍以上。经RCE诱导后,对抗生素、CHX和BBR产生交叉耐药的Sa菌株数分别是11,3和14。经BBR诱导后,对抗生素、CHX、RCE产生交叉耐药的Sa菌株数分别是9,2和14。结论:根据实验结果可知,抗生素、消毒剂和抗菌中药能诱导细菌产生交叉耐药,细菌对三者的抑菌机制可能相同。
[Abstract]:Aim: to study whether antibiotics, disinfectants (chlorhexidine) and anti-bacterial Chinese medicine (Coptis chinensis) can mediate cross-resistance of bacteria. Methods: the minimal inhibitory concentration (minimum inhibitory concentration,MIC) of the drug against staphylococcus aureus (Staphylococcus aureus,Sa) was determined according to the international standard broth dilution method. According to the results of resistance analysis, the sublethal concentration of bacteria was continuously cultured for 14 days to induce cross-resistance of bacteria. Results: the bacteria induced by ciprofloxacin (ciprofloxacin,CIP) and gentamicin (gentamicin,GEN) did not increase the resistance to chlorhexidine (chlorhexidine,CHX), but increased the sensitivity to CHX. One and four strains of Sa increased the resistance to (Rhizoma coptidis extract,RCE and berberine,BBR respectively. After induced by tetracycline (tetracycline,TET), the number of cross-resistant Sa strains to CHX,RCE and BBR were 3,6 and 8, respectively. After induction by CHX, almost all strains developed cross-resistance to at least one antibiotic, 7 strains and 9 strains respectively increased their resistance to RCE and BBR by more than four times. After induced by RCE, the number of cross-resistant Sa strains to antibiotics, CHX and BBR was 11,3 and 14. 5%, respectively. After induced by BBR, the number of Sa strains producing cross-resistance to antibiotics and CHX,RCE was 9, 2 and 14. 5%, respectively. Conclusion: according to the experimental results, antibiotics, disinfectants and antibacterial herbs can induce cross-resistance of bacteria, and the inhibition mechanism of bacteria to them may be the same.
【作者单位】：广西中医药大学药学院;
【基金】：广西区自然基金(编号:2014GXNSFAA118176,2012GXNSFAA276037) 南宁市科技计划资质项目(编号:20131062) 广西中医药大学2016年硕士研究生科研创新重点项目(编号:YJS201608)
【分类号】：R446.5

【相似文献】