建立D抗原免疫刺激T细胞活化模型的研究

发布时间：2019-05-14 00:52

【摘要】：目的了解RhD抗原免疫刺激途径,建立D抗原免疫刺激活化的模型。方法选取RhD血型不同的供者,采用红细胞与单个核细胞直接混合培养,以及DC细胞负载RhD抗原后与单个核细胞混合培养2种实验方案,流式细胞仪检测CD4~+和CD8~+T细胞的活化量。结果 RhD(-)个体的DC细胞负载D抗原后,可以有效活化RhD-个体的CD4~+和CD8~+T细胞,而直接混合培养方式则不能。结论本研究采用DC细胞负载RhD抗原的方法,成功诱导了RhD(-)个体的T细胞活化和增殖,验证了RhD抗原的胸腺依赖性抗原特性,建立了D抗原免疫刺激T细胞活化的模型,可以用于输血免疫的研究。
[Abstract]:Objective to investigate the immune stimulation pathway of RhD antigen and establish a model of D antigen immune stimulation activation. Methods the donors with different RhD blood groups were cultured directly with red blood cells and mononuclear cells, and DC cells were co-cultured with mononuclear cells loaded with RhD antigen. The activation of CD4~ and CD8~ T cells was detected by flow cytometry. Results the DC cells of RhD (-) individuals loaded with D antigen could effectively activate CD4~ and CD8~ T cells of RhD- individuals, but the direct mixed culture method could not. Conclusion the activation and proliferation of T cells in RhD (-) individuals were successfully induced by DC cells loaded with RhD antigen, the thymic dependent antigen characteristics of RhD antigen were verified, and the model of T cell activation stimulated by D antigen was established. It can be used in the study of blood transfusion immunity.
【作者单位】：深圳市血液中心;陕西省血液中心;Rh血型系统基础与临床研究关键技术攻关合作组;南方医科大学;
【基金】：深圳市科技计划项目(JCYJ20140403092619633)
【分类号】：R457.11

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