高灵敏性表面增强拉曼散射传感器的构建及其用于人体尿液中痕量甲醛的快速检测

发布时间：2019-05-28 05:35

【摘要】：目的:构建响应快速、灵敏度高的表面增强拉曼散射(surface-enhanced Raman scattering,SERS)传感器,实现对人体尿液中痕量甲醛的快速检测。方法:以对-氨基苯硫酚(p-ATP)为探针分子,通过表面自组装技术在银纳米粒子表面修饰具有SERS信号响应的单分子层,滴加甲醛后,甲醛通过醛基与p-ATP发生特异性缩合反应,p-ATP原有拉曼信号强度猝灭,进而实现对甲醛的间接检测。结果:甲醛在1.0×10~(-8)~1.0×10~(-7) mol/L范围内呈现良好的线性,相关系数为0.991 7,检测限为6.5×10~(-9) mol/L,回收率为97.5%~106.0%。结论:本方法可用于人体尿液中痕量甲醛的检测,具有耗时少、简便和灵敏度高等优点。
[Abstract]:Objective: to construct a surface enhanced Raman scattering (surface-enhanced Raman scattering,SERS) sensor with fast response and high sensitivity for the rapid determination of trace formaldehyde in human urine. Methods: the monolayer with SERS signal response was modified on the surface of silver nanoparticles by surface self-assembly technique with p-aminophenylthiophenol (p-ATP) as probe molecule. Formaldehyde reacted specifically with p-ATP through aldehyde group condensation reaction, and the original Raman signal intensity of p-ATP was quenched, and then the indirect detection of formaldehyde was realized. Results: formaldehyde showed a good linearity in the range of 1.0 脳 10 ~ (- 8) ~ 1.0 脳 10 ~ (- 7) mol/L, the correlation coefficient was 0.991, and the detection limit was 6.5 脳 10 ~ (- 9) mol/L,. The recovery was 97.5% 鈮，

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