外源化学物质对副溶血性弧菌药物敏感性的影响

发布时间：2019-06-05 15:39

【摘要】：【目的】细菌对抗生素的耐药性已成为全球公共卫生问题关注的热点。有研究表明外源添加化学物质可以增强耐药细菌对抗生素的敏感性。本研究比较了3种化学物质葡萄糖、丙氨酸、甘油对增强副溶血性弧菌抗生素敏感性的作用效果。【方法】在亚抑菌浓度抗生素胁迫条件下,通过比较副溶血性弧菌在添加终浓度为10 mmol/L葡萄糖、丙氨酸、甘油后细菌存活率随时间的变化水平,来观察弧菌对亚抑菌浓度抗生素敏感性作用效果的改变,并采用氧化磷酸化解偶联剂CCCP对实验结果进行验证。【结果】发现3种外源化学物质均能增强亚抑菌浓度氨基糖苷类抗生素对副溶血性弧菌的杀菌能力,其中外源添加葡萄糖对增强亚抑菌浓度卡那霉素的杀菌能力最为显著,而对其他种类抗生素的杀菌能力则无明显增强作用。加入氧化磷酸化解偶联剂CCCP后可消除由外源化学物质引发的弧菌抗生素敏感性作用增强的现象。【结论】通过调节细菌细胞代谢水平可提高耐药副溶血性弧菌对氨基糖苷类抗生素的敏感性,对多重耐药副溶血性弧菌的防控具有一定的实际应用价值。
[Abstract]:[objective] the resistance of bacteria to antibiotics has become the focus of global public health. Some studies have shown that exogenous addition of chemicals can enhance the sensitivity of drug-resistant bacteria to antibiotics. In this study, the effects of three chemicals glucose, alanine and glycerin on enhancing antibiotic sensitivity of Vibrio parahaemolyticus were compared. [methods] under the stress of subinhibitory concentration antibiotics, The effect of Vibrio parahaemolyticus on the sensitivity of Vibrio parahaemolyticus to subbacteriostatic concentration antibiotics was observed by comparing the changes of bacterial survival rate with time after the final concentration of 10 mmol/L glucose, alanine and glycerin was added to Vibrio parahaemolyticus. The experimental results were verified by oxidative phosphate desorption coupling agent CCCP. [results] it was found that all three exogenous chemicals could enhance the bactericidal ability of subinhibitory concentration aminoglycoside antibiotics against Vibrio parahaemolyticus. Among them, exogenous glucose had the most significant bactericidal ability to enhance the subinhibitory concentration of kanamycin, but had no significant effect on the bactericidal ability of other kinds of antibiotics. The addition of oxidative phosphate desorption coupling agent CCCP can eliminate the increase of antibiotic sensitivity of Vibrio parahaemolyticus caused by exogenous chemicals. [conclusion] by regulating the metabolic level of bacterial cells, the resistance of Vibrio parahaemolyticus to Vibrio parahaemolyticus can be improved. The sensitivity of aminoglycoside antibiotics, It has certain practical application value for the prevention and control of multi-drug resistant Vibrio parahaemolyticus.
【作者单位】：上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心;农业部水产品贮藏保鲜质量安全风险评估实验室;
【基金】：国家自然科学基金项目(No.31271870,31571917)~~
【分类号】：R446.5

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