超声造影对胃癌患者病灶中癌细胞增殖、侵袭功能及血管新生程度的评估价值

发布时间：2019-07-02 09:49

【摘要】：目的:探讨超声造影对胃癌患者病灶中癌细胞增殖、侵袭功能及血管新生程度的评估价值。方法:纳入2012年8月~2016年5月本院收治的胃癌患者39例作为胃癌组,胃溃疡患者48例作为胃溃疡组,另取同期在本院接受胃镜检查的健康人50例作为正常对照组。入院后当日,应用彩色多普勒超声仪检测胃部超声造影参数;采用荧光定量PCR法检测胃部组织增殖、侵袭基因mRNA表达量;采用酶联免疫吸附法(ELISA)检测血清血管新生指标含量。结果:胃癌组、胃溃疡组患者的超声造影参数增强时间(ET)、峰值时间(TTP)显著低于正常对照组,胃癌组患者的超声造影参数ET、TTP显著低于胃溃疡组;胃癌组患者组织中Stat3、Survivin、Bcl-2、β-catenin、eIF4E、CD44、UHRF1、c-met的mRNA表达量以及血清中血管内皮生长因子(VEGF)、表皮生长因子受体(EGFR)、低氧诱导因子-1α(HIF-α)、血管生成素-2(Ang-2)的含量均高于胃溃疡组和正常对照组,组织中E-cadherin的mRNA表达量低于胃溃疡组和正常对照组;Spearman相关性分析显示,超声造影参数ET、TTP水平与病灶中癌细胞增殖、侵袭功能及血管新生指标呈相关关系。结论:超声造影参数可准确评估胃癌恶性程度,有望成为日后胃癌早期诊断及治疗指导的可靠手段。
[Abstract]:Objective: to evaluate the value of contrast-enhanced ultrasound in evaluating the proliferation, invasion and neovascularization of cancer cells in patients with gastric cancer. Methods: from August 2012 to May 2016, 39 patients with gastric cancer were enrolled as gastric cancer group, 48 patients with gastric ulcer as gastric ulcer group, and 50 healthy people who underwent gastroscopy in our hospital as normal control group. On the day after admission, the parameters of gastric contrast-enhanced ultrasound were measured by color Doppler ultrasound, the proliferation of gastric tissue and the expression of invasive gene mRNA were detected by fluorescence quantitative PCR, and the content of serum angiogenic indexes was detected by enzyme-linked immunosorbent assay (ELISA). Results: the contrast-enhanced time (TTP) of contrast-enhanced time (TTP) in gastric cancer group and gastric ulcer group was significantly lower than that in normal control group, and the contrast-enhanced time (ET,TTP) in gastric cancer group was significantly lower than that in gastric ulcer group. The mRNA expression of Stat3,Survivin,Bcl-2, 尾-catenin,eIF4E,CD44,UHRF1,c-met and the contents of vascular endothelial growth factor (VEGF), (EGFR), hypoxia inducer-1 伪 (HIF- 伪) and angietin-2 (Ang-2) in gastric cancer group were higher than those in gastric ulcer group and normal control group, and the mRNA expression of E-cadherin in gastric ulcer group and normal control group was lower than that in gastric ulcer group and normal control group. Spearman correlation analysis showed that the ET,TTP level of contrast-enhanced ultrasound was correlated with the proliferation, invasive function and angiogenic indexes of cancer cells in the lesion. Conclusion: contrast-enhanced ultrasound can accurately evaluate the malignant degree of gastric cancer and is expected to be a reliable method for early diagnosis and treatment of gastric cancer in the future.
【作者单位】：四川省遂宁市中医院功能科;
【基金】：四川省科技攻关计划项目(2005sz01357)~~
【分类号】：R735.2;R445.1

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