反相高效液相色谱法同时测定新生儿尿液中4种苯丙酮尿症特征酸的含量
发布时间：2019-08-14 14:58
【摘要】：提出了反相高效液相色谱同时测定新生儿尿液中4-羟基苯乙酸、2-羟基苯乙酸、苯乙酸和苯丙酮酸等4种苯丙酮尿症特征酸的方法。取尿液样品5.00mL,乙腈5mL,混合振荡1min,离心10min,取上清液进行液相色谱分离。用Waters Symmetry C18色谱柱作为固定相,用不同比例的乙腈和0.15%(体积分数)磷酸溶液进行梯度洗脱,检测波长为210,289nm。在上述条件下,4种特征酸分离完全,并且不受尿液中内源性物质的干扰。4种特征酸的质量浓度在一定范围内呈线性,检出限(3S/N)分别为0.43,0.22,0.62,0.42mg·L-1。按标准加入法在3个浓度水平上进行加标回收试验,测得回收率在89.1%~108%之间,测定值的相对标准偏差(n=6)小于4%。
[Abstract]:A reverse-phase high performance liquid chromatography for the determination of 4-hydroxyphenylacetic acid,2-hydroxyphenylacetic acid, phenylacetic acid and phenylacetone acid in the urine of the newborn was presented. 5.00 mL of urine sample,5 mL of acetonitrile,1 min of mixing, and 10 min for centrifugation, and the supernatant was separated by liquid chromatography. The gradient elution was performed with a Waters Symmetry C18 column as a stationary phase with a different ratio of acetonitrile and 0.15% (volume fraction) phosphoric acid solution, with a detection wavelength of 210,289 nm. Under the above conditions,4 kinds of characteristic acid were separated completely and not interfered by endogenous substances in urine. The mass concentration of 4 kinds of characteristic acids was linear in a certain range, and the detection limit (3S/ N) was 0.43, 0.22, 0.62 and 0.42 mg 路 L-1, respectively. The recoveries were found to be between 89.1% and 108%, and the relative standard deviation (n = 6) of the measured values was less than 4%.
【作者单位】： 浙江工业大学化学工程学院;
【基金】：浙江省自然科学基金资助项目(M203030;Y4110536)
【分类号】：R446.12;O657.72
[Abstract]:A reverse-phase high performance liquid chromatography for the determination of 4-hydroxyphenylacetic acid,2-hydroxyphenylacetic acid, phenylacetic acid and phenylacetone acid in the urine of the newborn was presented. 5.00 mL of urine sample,5 mL of acetonitrile,1 min of mixing, and 10 min for centrifugation, and the supernatant was separated by liquid chromatography. The gradient elution was performed with a Waters Symmetry C18 column as a stationary phase with a different ratio of acetonitrile and 0.15% (volume fraction) phosphoric acid solution, with a detection wavelength of 210,289 nm. Under the above conditions,4 kinds of characteristic acid were separated completely and not interfered by endogenous substances in urine. The mass concentration of 4 kinds of characteristic acids was linear in a certain range, and the detection limit (3S/ N) was 0.43, 0.22, 0.62 and 0.42 mg 路 L-1, respectively. The recoveries were found to be between 89.1% and 108%, and the relative standard deviation (n = 6) of the measured values was less than 4%.
【作者单位】： 浙江工业大学化学工程学院;
【基金】：浙江省自然科学基金资助项目(M203030;Y4110536)
【分类号】：R446.12;O657.72
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