Cryopreservation of Seeds:Dormancy and Water Content in Pers

发布时间：2024-02-22 20:44

　　本研究的目的是为了解决种子在基因库标准和低温储藏下的一些关键问题,主要包括休眠和水分含量两个方面的研究。尽管在低温贮藏中,种子的休眠和水分含量是可控的,但是对于长期保存还是比较难控制的。第2章主要研究车桑子种子的物理休眠和低温保存的可行性。更详细地说是研究在种子保存过程中何时和用哪种方式解除物理休眠。第3章将第2章的内容扩展到杏仁种子的研究中,该部分的研究可以应用于其他蔷薇科物种和具有坚实内果皮的种子。第4和5章主要研究了莴苣种子的贮藏,为其他异常型种子的保存提供了借鉴。第4章主要从降温速率上切入优化了种子的保存条件。第5章主要研究了低温保护剂对种子保存的影响。主要内容如下:本章将对种子的物理休眠和低温贮藏的可行性进行研究。采用车桑子(Sapindaceae)种子为研究对象,该种子具有不透水的种皮且进行物理休眠。目前,已有的研究可以在实验室的条件下可以对种子进行机械划痕、干燥、硫酸和热水处理来改变种子的通透性从而解读种子的休眠释放机理,但是在自然条件下该机理还未被完全解读。本章的主要研究内容是理解车桑子种子在人工填埋2年后的休眠释放机理。此外,本文还设计了种子的低温贮藏程序。新鲜的种子...

【文章页数】：113 页

【学位级别】：博士

【文章目录】：
摘要
ABSTRACT
Abbreviations
Chapter 1 Introduction
    1.1 SEED STORAGE
        1.1.1 Types of seeds
        1.1.2 Usefulness of discrete classification system
        1.1.3 Seed storage conditions
        1.1.4 Selecting and treating seeds for storage
        1.1.5 Drying seeds – importance of drying rate
        1.1.6 Cryoprotectants
    1.2 FREEZING OF IMBIBED SEEDS IN NATURAL ENVIRONMENT
        1.2.1 Freezing avoidance-supercooling
        1.2.2 Freezing tolerance by freeze-desiccation
    1.3 STATE OF WATER IN SEEDS
    1.4 USING ORTHODOX SEEDS AT IMBIBED STATE AS A MODEL FOR RECALCITRANT SPECIES
    1.5 DORMANCY
    1.6 OUTLINE OF THE CURRENT PROJECT
Chapter 2 Dormancy and cryopreservation of Dodonea viscosa seeds
    2.1 INTRDOUCTION
    2.2 MATERIALS AND METHODS
        2.2.1 Seed collection
        2.2.2 Moisture content measurement
        2.2.3 Germination test
        2.2.4 Dormancy breaking
        2.2.5 Seed imbibition
        2.2.6 Cryopreservation
        2.2.7 Statistical analysis
    2.3 RESULTS
        2.3.1 Germination and moisture content of freshly collected seed
        2.3.2 Seed imbibition
        2.3.3 Effect of dry storage on dormancy breaking
        2.3.4 Effect of different temperatures on breaking dormancy
        2.3.5 Importance of hot-water temperature in breaking dormancy
        2.3.6 Cryopreservation of seeds
    2.4 DISCUSSION
Chapter 3 Dormancy and cryopreservation of Apricot (Prunus armeniaca) seeds fromXinjiang province
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Seed materials
        3.2.2 Seed desiccation and moisture content determination
        3.2.3 Germination test
        3.2.4. Imbibition of water
        3.2.5 Cold stratification
        3.2.6 Effect of GA3 on dormancy break
        3.2.7 Effect of GA3 in endocarp intact seeds
        3.2.8 Cryopreservation of seeds
        3.2.9 Statistical analysis
    3.3 RESULTS
        3.3.1 Moisture content of the seeds
        3.3.2 Germination of seeds
        3.3.3 Imbibition of water
        3.3.4 Effect of GA3 on dormancy break
        3.3.5 Effect of GA3 in endocarp intact seeds
        3.3.6 Cold stratification
        3.3.7 Seed desiccation and germination
        3.3.8 Cryopreservation of seeds
    3.4 DISCUSSION
Chapter 4 Cryopreservation of hydrated lettuce (Lactuca sativa L.) seeds
    4.1 INTRODUCTION
    4.2 MATERIALS AND METHODS
        4.2.1 Seed material and germination conditions
        4.2.2 Seed imbibition and moisture content determination
        4.2.3 Thermal analyses
        4.2.4 Controlled cooling of seeds
        4.2.5 Liquid nitrogen storage
        4.2.6 Use of pierced seeds
        4.2.7 Statistical analysis
    4.3 RESULTS
    4.4 DISCUSSION
Chapter 5 Effects of dimethyl sulfoxide concentration, pre-cooling and cooling rate oncryopreservation of hydrated lettuce seeds
    5.1 INTRODUCTION
    5.2 MATERIALS AND METHODS
        5.2.1 Seed materials
        5.2.2 Seed germination
        5.2.3 Moisture content assessment
        5.2.4 Seed imbibition
        5.2.5 DMSO treatment
        5.2.6. Freezing treatments and differential thermal analysis (DTA)
        5.2.7 Statistical analysis
    5.3 RESULTS
    5.4. DISCUSSION
Chapter 6 Summary of main findings
References
List of papers published or in progress duringthe post graduate study
Acknowledgements



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