硫砷金精矿的嗜铁钩端螺旋菌生物氧化预处理研究

发布时间：2024-05-15 23:31

　　生物冶金是指在相关微生物存在时由于微生物的催化氧化作用将矿物中有价金属以离子形式溶解到浸出液中加以回收或将矿物中有害元素溶解并除去的方法。因其处理成本相对较低、环境污染小、反应温和等优点、在难处理金矿预处理过程中得到广泛关注。本研究将嗜铁钩端螺旋菌作为预处理用微生物,系统研究其与硫砷金精矿的生物氧化过程。首先研究嗜铁钩端螺旋菌ATCC 49881在9K培养基中的生长情况,确定最优生物氧化活性的生长条件,得出以下结论：1.嗜铁钩端螺旋菌ATCC 49881在9K培养基中的生长情况表明：不同初始pH值下,嗜铁钩端螺旋菌ATCC 49881指数期发生在12h至50h,在培养时间为97h时,嗜铁钩端螺旋菌菌体密度达到最大(约8.2 x 106细胞/ml、5.6 x 106细胞/ml、6.4 x 106细胞/ml为初始pH值分别为1.6、pH值1.8和pH为2.0)。2.通过使用Lineweaver burk进行拟合得到嗜铁钩端螺旋菌的莫诺方程。由莫诺方程可知,当初始pH值为1.6、pH值1.8和pH值2.0时、菌体的最大比生长速率(μmax)分别为0.076 h-1、0.178 h-1、0.0...

【文章页数】：93 页

【学位级别】：硕士

【文章目录】：
摘要
ABSTRACT
CHAPTER Ⅰ INTRODUCTION
    1.1 Background
        1.1.1 Bioleaching
        1.1.2 Development of bioleaching
        1.1.3 Development of bioleaching in China
    1.2 Brief description of gold ore
        1.2.1 Refractory of gold ore
        1.2.2 Factor affecting leaching performance
    1.3 Bioleaching and biooxidation mechanisms
    1.4 Major species of microorganisms in bioleaching
        1.4.1 Characteristic of Leptospirillum ferriphium
    1.5 Microbial growth and its dynamic
    1.6 Basic concept on microbial kinetics of iron oxidation
    1.7 Basic concept on microbial kinetics of arsenopyrite oxidation
    1.8 Aims and Objective of Research
        1.8.1 Aims and objectives
        1.8.2 Research scope
CHAPTER Ⅱ MATERIALS AND METHODS
    2.1 Materials
        2.1.1 Arsenopyrite-bearing gold concentrate characterization
    2.2 Equipment and chemical reagents
        2.2.1 Equipment
        2.2.2 Chemical reagents
    2.3 Methodology of research
        2.3.1 Experimental procedures
        2.3.2 Experimental apparatus
        2.3.3 Microbial culture
    2.4 Analytical Technique
        2.4.1 Determination of bacterial cell density by direct microscopic counting
        2.4.2 pH and oxidation reduction potential
        2.4.3 Spectrophotometric of iron assay
        2.4.4 Total dissolved arsenic assay
    2.5 Sample collections and data handling
CHAPTER Ⅲ MICROBIAL ACTIVATION AND MICROBIAL GROWTH
    3.1 Leptospirillum ferriphilum activation
    3.2 Leptospirillum ferriphilum growth
    3.3 The kinetics study
        3.3.1 Cell growth kinetics
        3.3.2 Kinetics of ferrous iron biooxidation
    3.4 Summary
CHAPTER Ⅳ BIOOXIDATION EXPERIMENTS OFARSENOPYRITE-BEARING GOLD CONCENTRATE
    4.1 Preliminary bioleaching test
    4.2 Biooxidation optimization
        4.2.1 Pretreatment of arsenopyrite-bearing gold concentrate with variation of chemical compound
        4.2.2 Pretreatment of arsenopyrite-bearing gold concentrate with variation of ferrous iron(Fe²⁺)
        4.2.3 Pretreatment of arsenopyrite-bearing gold concentrate with variation of ferric iron(Fe³⁺)
    4.3 The kinetics study of the biooxidation of arsenopyrite-bearing gold concentrate
    4.4 Summary
CHAPTER Ⅴ CLOSURE
    5.1 Conclusion
    5.2 Suggestions
REFERENCES
APPENDICES
致谢
攻读硕士学位期间发表的学术论文目录



本文编号：3974366