固相碳源脱氮同步去除壬基酚的试验研究

发布时间：2018-04-05 17:15

本文选题：固相碳源　切入点：同步硝化反硝化　出处：《中国环境科学》2017年08期

【摘要】：采用一种基于PHBV研发的新型固相碳源NS,构建同步硝化反硝化脱氮生物反应器,同时探究常见的内分泌干扰物壬基酚(NP)在系统中的同步去除特性,并运用Illumina Mi Seq高通量测序和荧光定量PCR(q PCR)技术,对微生物群落进行解析.反应器运行效果良好,硝酸盐氮和氨氮去除率可分别达到96.18%和82.54%,出水COD平均浓度为27.48mg/L,壬基酚平均去除率为81.17%.高通量测序结果表明,Dechloromonas(脱氯单胞菌属)和Zoogloea(动胶菌属)等报道的具脱氮作用的菌属在无壬基酚条件下占据优势地位.加入壬基酚的条件下,菌种总数和α多样性降低.荧光定量PCR结果表明,系统中氮循环基因丰度较高,nir S基因丰度最大,而amo A基因丰度最低.进水中存在壬基酚的条件下碳源表面nir S基因丰度较低.
[Abstract]:A novel solid-phase carbon source based on PHBV was used to construct a simultaneous nitrification-denitrification and denitrification bioreactor and to explore the synchronous removal characteristics of common endocrine disruptor nonylphenol in the system.The microbial community was analyzed by Illumina Mi Seq high throughput sequencing and fluorescence quantitative PCR(q PCR.The removal rate of nitrate nitrogen and ammonia nitrogen was 96.18% and 82.54% respectively. The average concentration of effluent COD was 27.48 mg / L and the average removal rate of nonylphenol was 81.17%.The results of high throughput sequencing showed that Dechloromonas (Dechloromonas) and Zoogloea (Actinomycetes) were dominant in denitrification under the condition of nonylphenol.When nonylphenol was added, the total number of bacteria and 伪 -diversity decreased.The results of fluorescence quantitative PCR showed that the nitrogen cycle gene abundance was higher in the system than that in the amo A gene, and the highest abundance was found in the Nir S gene and the lowest in the amo A gene.The abundance of nir S gene on the surface of carbon source was lower under the condition of nonylphenol in the influent.
【作者单位】：北京大学环境科学与工程学院;
【基金】：国家自然科学基金资助项目(51378021) 国家水体污染控制与治理重大科技专项(2012ZX07102002)
【分类号】：X701

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