甜高粱在重金属镉胁迫下的生理反应和基因鉴定
发布时间：2023-05-09 21:49
当今社会,工业发展和人类活动导致大量的土地受重金属污染。农业区过多的重金属,例如,铅(Pb)、铬(Cr)、锌(Zn)、镉(Cd)、铜(Cu)和镍(Ni)等严重威胁食品安全和公共健康。为了阻止人类和动物遭受毒害,消除或者修复土壤重金属污染是迫切需要的。镉是一种有毒且非必须的重金属,很容易被植物吸收并积累在农产品中。目前,有很多防止、消除和修复环境中污染物负面效应的方法和过程,但是对于此目的的应用很难实施或者根本无法实施。因此,所有的研究应考虑利用生物因素来处理和修复环境。植物修复被定义为利用植物消除环境中的污染物或者减少它们的危害。这个过程在不对环境造成干扰的同时还有一定的经济效益。高粱(Sorghum bicolor L.)是一种惠民的有多重用途的农作物,在农业生态系统中可以作为粮食、饲料、纤维和燃料。甜高粱包含了高粱的不同的栽培品种的有点,且茎中储存大量的蔗糖以及有很大的生物量,因此可以作为生物燃料,而糖含量确定了甜高粱作为生物燃料的潜力。甜高粱多汁的茎通常含有大约16-18%的可发酵糖和大量的无结构性糖类。为了初步评估甜高粱植物修复的潜力,以前的研究已对重金属(Cd,Pb,Zn,C...
【文章页数】：149 页
【学位级别】：博士
【文章目录】：
摘要
ABSTRACT
CHAPTER I LITERATURE REVIEW
1.1 Heavy metals in soils and plants
1.1.1 Contaminated heavy metal soil
1.1.2 Cadmium in the soil-plant relationship
1.1.3 Mechanism of uptake heavy metal into plant
1.2 Phytoremediation
1.2.1 Detoxification mechanisms in plants
1.2.2 Using plant for phytoremediation
1.3 Introduction about sorghum
1.3.1 Sweet sorghum and application value
1.3.2 Respones of sweet sorghum to heavy metal
1.4 Principles of transcriptome analysis and gene expression quantification
1.4.1 m RNA seq- transcriptome
1.4.2 Identification of gene expression of plant responses to adverseenvironmental condition
CHAPTER II PHOTOSYNTHESIS AND GROWTH RESPONSE OF SWEET SORGHUM PLANTSTO DIFFERENT LEVELS OF HEAVY METAL CADMIUM
2.1 Introduction
2.2 Material and methods
2.2.1 Plant materials and growth conditions
2.2.2 Growth characteristic analysis
2.2.3 Photosynthesis analysis
2.2.4 Data analysis
2.3 Results
2.3.1 Growth characteristic
2.3.2 Effect of Cd2+ on plant photosynthesis parameters
2.4 Discussion
2.5 Conclusions
CHAPTER III CADMIUM ACCUMULATION IN PLANT AND EFFECT ON NUTRIENTELEMENTS
3.1 Introduction
3.2 Material and methods
3.2.1 Plant materials and growth conditions
3.2.2 Sampling methods
3.2.3 Measurement of metal concentration
3.2.4 Data analysis
3.3 Result
3.3.1 Cd concentration in organs of sweet sorghum at the seedling stage .. 50
3.3.2 Cd concentration in organs of sweet sorghum at the hard dough stage
3.3.3 Effects of cadmium on uptake and translocation of nutrient elements
3.4 Discussion
3.4.1 Distribution of Cd in sweet sorghum
3.4.2 Effect of Cd on nutrient elements transport in plant
3.5 Conclusions
CHAPTER IV CADMIUM INDUCED MODIFICATION OF SUGAR ACCUMULATION ANDTRANSLOCATION ALONG SWEET SORGHUM STEM
4.1 Introduction
4.2 Material and methods
4.2.1 Plant materials and growth conditions
4.2.2 Measure sugar content
4.2.3 Data analysis
4.3 Results
4.3.1 Cd effect on total soluble solid in stem of sweet sorghum
4.3.2 The relationship between Cd concentration and and Brix
4.3.3 Cd effect on Soluble Sugar in stem of sweet sorghum
4.4 Discussion
4.4.1 Cd effect on Brix
4.4.2 Cd effect on soluble sugar
4.5 Conclusions
CHAPTER V TRANSCRIPTOME ANALYSIS OF SWEET SORGHUM IN RESPONSE TOCADMIUM STRESSES
5.1 Introduction
5.2 Material and methods
5.2.1 Plant Growth Conditions
5.2.2 Harvesting plant material
5.2.3 Sequencing Protocol
5.3 Results
5.3.1 Isolation of Total RNA results
5.3.2 Illumina Sequencing and the Assembly of Sequence Reads
5.3.3 Differential Expression Analysis
5.3.4 Gene Ontology (GO) Functional Enrichment Analysis for the DEGs
5.3.5 KEGG pathway enrichment analysis of differential gene
5.3.6 Functional analyses of differentially expressed genes
5.4 Conclusions
CONCLUSSION AND OUTLOOK
REFERENCES
Acknowledgements
RESEARCH ARTICLES PUBLISHED
本文编号：3812438
【文章页数】：149 页
【学位级别】：博士
【文章目录】：
摘要
ABSTRACT
CHAPTER I LITERATURE REVIEW
1.1 Heavy metals in soils and plants
1.1.1 Contaminated heavy metal soil
1.1.2 Cadmium in the soil-plant relationship
1.1.3 Mechanism of uptake heavy metal into plant
1.2 Phytoremediation
1.2.1 Detoxification mechanisms in plants
1.2.2 Using plant for phytoremediation
1.3 Introduction about sorghum
1.3.1 Sweet sorghum and application value
1.3.2 Respones of sweet sorghum to heavy metal
1.4 Principles of transcriptome analysis and gene expression quantification
1.4.1 m RNA seq- transcriptome
1.4.2 Identification of gene expression of plant responses to adverseenvironmental condition
CHAPTER II PHOTOSYNTHESIS AND GROWTH RESPONSE OF SWEET SORGHUM PLANTSTO DIFFERENT LEVELS OF HEAVY METAL CADMIUM
2.1 Introduction
2.2 Material and methods
2.2.1 Plant materials and growth conditions
2.2.2 Growth characteristic analysis
2.2.3 Photosynthesis analysis
2.2.4 Data analysis
2.3 Results
2.3.1 Growth characteristic
2.3.2 Effect of Cd2+ on plant photosynthesis parameters
2.4 Discussion
2.5 Conclusions
CHAPTER III CADMIUM ACCUMULATION IN PLANT AND EFFECT ON NUTRIENTELEMENTS
3.1 Introduction
3.2 Material and methods
3.2.1 Plant materials and growth conditions
3.2.2 Sampling methods
3.2.3 Measurement of metal concentration
3.2.4 Data analysis
3.3 Result
3.3.1 Cd concentration in organs of sweet sorghum at the seedling stage .. 50
3.3.2 Cd concentration in organs of sweet sorghum at the hard dough stage
3.3.3 Effects of cadmium on uptake and translocation of nutrient elements
3.4 Discussion
3.4.1 Distribution of Cd in sweet sorghum
3.4.2 Effect of Cd on nutrient elements transport in plant
3.5 Conclusions
CHAPTER IV CADMIUM INDUCED MODIFICATION OF SUGAR ACCUMULATION ANDTRANSLOCATION ALONG SWEET SORGHUM STEM
4.1 Introduction
4.2 Material and methods
4.2.1 Plant materials and growth conditions
4.2.2 Measure sugar content
4.2.3 Data analysis
4.3 Results
4.3.1 Cd effect on total soluble solid in stem of sweet sorghum
4.3.2 The relationship between Cd concentration and and Brix
4.3.3 Cd effect on Soluble Sugar in stem of sweet sorghum
4.4 Discussion
4.4.1 Cd effect on Brix
4.4.2 Cd effect on soluble sugar
4.5 Conclusions
CHAPTER V TRANSCRIPTOME ANALYSIS OF SWEET SORGHUM IN RESPONSE TOCADMIUM STRESSES
5.1 Introduction
5.2 Material and methods
5.2.1 Plant Growth Conditions
5.2.2 Harvesting plant material
5.2.3 Sequencing Protocol
5.3 Results
5.3.1 Isolation of Total RNA results
5.3.2 Illumina Sequencing and the Assembly of Sequence Reads
5.3.3 Differential Expression Analysis
5.3.4 Gene Ontology (GO) Functional Enrichment Analysis for the DEGs
5.3.5 KEGG pathway enrichment analysis of differential gene
5.3.6 Functional analyses of differentially expressed genes
5.4 Conclusions
CONCLUSSION AND OUTLOOK
REFERENCES
Acknowledgements
RESEARCH ARTICLES PUBLISHED
本文编号：3812438
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