Alleviation of Copper-induced Seed Germination Inhibition an

发布时间：2023-10-21 13:19

　　

【文章页数】：78 页

【学位级别】：硕士

【文章目录】：
ABSTRACT
LIST OF ABBREVIATIONS
CHAPTER 1 LITERATURE REVIEW
    1.1 Advances of copper in plants
        1.1.1 Cu deficiency in plant
            1.1.1.2 Symptoms of Cu deficiency
        1.1.2 Cu toxicity in plants
        1.1.3 Tolerance mechanism of plants against Cu toxicity
    1.2 Advances in reactive oxygen species research
        1.2.1 Generation of ROS in plants
        1.2.2 Role of antioxidant in scavenging ROS
    1.3 Advances in methane research
        1.3.1 Characteristics of methane
        1.3.2 Advances of CH₄ in animals and plants
        1.3.3 Sources of methane
            1.3.3.1 Microbial production of CH4

            1.3.3.2 Non-microbial production of CH₄

        1.3.4 Environmental stress factors and CH₄

    1.4 Advances of proline in plant research
        1.4.2 Biosynthesis of proline
        1.4.3 Effect of proline on antioxidant system and oxidative stress
            1.4.3.1 Effect of proline under salinity stress in plants
            1.4.3.2 Effect of proline under radiation stress in plants
            1.4.3.3 Effect of proline under temperature stress in plants
            1.4.3.4 Effect of proline on other enzymes and metabolites
            1.4.3.5 Effect of Proline under heavy metal stress in plants
        1.4.4 Toxicity of proline
CHAPTER 2 MATERIALS AND METHODS
    2.1 Plant materials, growth conditions, and experimental design
    2.2 Preparation of methane-rich water (MRW)
        2.2.1 Estimation of endogenous CH₄ content
    2.3 Assay of amylase activity and total soluble sugar contents
    2.4 Determination of thiobarbituric acid reactive substances (TBARS)
    2.5 Histochemical analyses
    2.6 Enzyme extraction and assays
    2.7 Gel electrophoresis
    2.8 Real-time quantitative RT-PCR analysis
    2.9 Determination of proline content
    2.10 Determination of Cu content
    2.11 Statistical analysis
CHAPTER 3 RESULTS
    3.1 Inhibition of germination and seedling growth in alfalfa plant by Cu
        3.1.1 Effect of MRW on the alleviation of Cu stress in alfalfa plants
    3.2 Endogenous CH4 content is increasing in alfalfa seedlings upon Cu stress
    3.3 Effect of MRW on amylase activities and total sugar contents
    3.4 Effect of MRW on TBARS contents
    3.5 Effect of MRW on antioxidant enzymes/genes
    3.6 Effect of MRW on proline accumulation under Cu stress
    3.7 Effect of MRW and Cu on the P5CS activity and PDH transcript level
    3.8 Effect of MRW on Cu concentration
CHAPTER 4 DISCUSSION
Conclusion
REFERENCES
LIST OF PUBLICATIONS
ACKNOWLEDGEMENT



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