The open field test as a method for ethology in Kunming mice

  本文关键词：旷场实验，由笔耕文化传播整理发布。

摘要：

目的 探讨旷场实验作为昆明小鼠行为学评价方法的重测信度.方法 成年雄性KM小鼠放入旷场箱正中央格,摄像系统记录5 min的行为变化,实验间隔1周进行2次;通过组内相关系数(ICC)和一致性系数(Kappa)对旷场实验进行重测信度检验,参数如下:中央格数、外周格数、中央格百分比、水平运动、垂直运动、总得分及粪便粒数.结果 ①ICC结果表明,除粪便粒数(ICC=0.376,P＜0.05)外,中央格数(ICC=0.578,P＜0.01)、外周格数(ICC=0.615,P＜0.01)、中央格百分比(ICC=0.523,P＜0.01)、水平运动(ICC=0.607,P＜0.01)、垂直运动(ICC=0.606,P＜0.01)及总得分(ICC=0.902,P＜0.01)均显示具有良好重测信度,尤其是总得分初测与重测具有良好相关性(r=0.815,P＜0.01).②Kappa结果表明,仅总得分(Kappa=0.680,P＜0.01)显示OFT具有良好重测信度.③t检验结果表明,外周格数(t=3.01,P＜0.01)、水平运动(t=3.34,P＜0.01)和总得分(t=7.86,P＜0.001)初测与重测结果差异具有统计学意义.结论 总得分可以作为昆明小鼠OFT的一个稳定参数,具有较好的重测信度.

Abstract： 

Objective To explore the test-retest reliability of the open field test,as a method for ethology in Kunming mice. Methods The behavior of adult male Kunming mice in the open field test was recorded twice,once for five minutes, with a one-week inter-trial interval. The following parameters were evaluated:number of central squares; number of peripheral squares; percent of central squares; number of horizontal squares; number of rearings; total score; and number of fecal boli. Subsequently,the intraclass correlation coefficient (ICC) and the agreement index (Kappa) were calculated for each of these parameters. Results Both ICC and Kappa for total score( ICC =0.902, P＜0.01; Kappa =0.680, P＜0.01; r=0.815, P＜0.01 ) showed a good reliability and correlation between the test trials. Only ICC for number of central squares ( ICC = 0. 578, P ＜ 0.01 ), number of peripheral squares( ICC =0.615, P＜0.01 ) ,percent of central squares(ICC=0.523, P＜0.01) ,number of horizontal squares( ICC = 0. 607, P ＜ 0.01 ) and number of rearings ( ICC = 0. 606, P ＜ 0.01 ) indicated a fair reliability between the test trials. Neither ICC nor Kappa for number of fecal boli displayed a significant reliability between the test trials. The difference of these parameters between test and retest, such as number of peripheral squares( t =3.01, P＜0.01 ),number of horizontal squares( t = 3.34, P ＜ 0.01 ) and total score( t = 7.86, P ＜ 0.01 ) were significant. Conclusion The parameter,total score for Kunming mice in the open field test,is found to be stable over time, and indicates a good reliability with a one-week inter-trial interval.

  本文关键词：旷场实验，，由笔耕文化传播整理发布。