地果的转录组学分析(英文)
发布时间：2024-11-02 01:25
为探究地果(Ficus tikoua)的遗传变异特征,利用Illumina HiSeq-2500平台获取地果的基因表达谱。结果表明,共获得了197 362个转录本,总长度和平均长度分别为114 072 125和577 bp。使用BlastX和BlastN共注释了139 992个转录本(占总数的70.93%)。从3个品种叶片和茎的6对样品中,分别鉴定了12 397、12 340、10 373、94 431、71 830和44 465个差异表达基因,以及注释了126、129、125、134、138和137条代谢途径。这将有助于理解地果的遗传特征,以及不同组织中代谢途径的变化。
【文章页数】：12 页
【文章目录】：
1 Materials and methods
1.1 Plant materials
1.2 RNA-Seq
1.3 de novo Assembly and functional annotation
1.4 Gene expression analysis
1.5 CDS predication
1.6 SSR and SNP analysis
2 Results
2.1 RNA sequencing and de novo assembly
2.2 Functional annotation and classification
2.3 Prediction of coding sequences(CDSs)
2.4 Analysis of differential gene expression
2.5 GO enrichment analysis of DEGs
2.6 KEGG enrichment analysis of DEGs
2.7 SNPs and SSRs in the transcriptome library
3 Discussion
本文编号：4008802
【文章页数】：12 页
【文章目录】：
1 Materials and methods
1.1 Plant materials
1.2 RNA-Seq
1.3 de novo Assembly and functional annotation
1.4 Gene expression analysis
1.5 CDS predication
1.6 SSR and SNP analysis
2 Results
2.1 RNA sequencing and de novo assembly
2.2 Functional annotation and classification
2.3 Prediction of coding sequences(CDSs)
2.4 Analysis of differential gene expression
2.5 GO enrichment analysis of DEGs
2.6 KEGG enrichment analysis of DEGs
2.7 SNPs and SSRs in the transcriptome library
3 Discussion
本文编号：4008802
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