Products Cloning Gene Analysis
本文关键词：Mutagenesis，由笔耕文化传播整理发布。
Cloninig & Gene Analysis
The detailed information can be referred in the following linked sites.
Applications Product Name
DNA fragment purification
High efficient ligation
High efficient blunting
TA cloning of PCR products having overhanged dA at the 3'-ends.
PCR products from Taq DNA polymerase, Blend Taq [Code No. BTQ-101],
Blend Taq -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101]
are available.
TA cloning of blunt-end PCR products amplified using
KOD -Plus- [Code No. KOD-201] or KOD FX [Code No. KFX-101].
Attachment of dA at the 3' - ends of blunt end PCR products
from KOD -Plus- and KOD FX ( For TA cloning )
Site-directed Mutagenesis
Southern & Northern hybridization
100 bp DNA Ladder Marker
1 kb DNA Ladder Marker
DNA fragment purification
High Efficient DNA Fragment purification Kit
MagExtractor
-PCR & Gel Clean up- (click here)Code No. NPK-601 200 preparation
MagExtractor -PCR & Gel Clean up- provides a simple and reliable method for the rapid purification of DNA fragments from a PCR solution, enzyme solution, or agarose gel slices utilizing magnetic silica beads. This kit is based on binding properties of DNA to a silica surface in the presence of chaotropic agents. The purified DNA fragments can be used directly for general molecular biology experiments.
High efficient ligation
Ready-to-use ligation reagent
Ligation high Ver.2
(click here)
Code No. LGK-201 750 µl (100 reactions)
The ligation reaction is an essential step in genetic recombination experiments. For this reaction, T4 DNA ligase has been widely used. Ligation high Ver.2 is a highly efficient premixed T4 Ligase reagent.
Reagents at -20ºC
Left: conventional, Right: Ligation high Ver.2
High efficient blunting
Highly efficient blunting reagent
Blunting high
(click here)Code No. BLK-101 20 reactions
Blunting high is a kit that produces a blunt end at the DNA terminus, and allows for its use in a subsequent ligation step using KOD DNA polymerase1 and DNA ligase reagents, respectively.
High efficient TA cloning kit
TArget Clone
(click here)
TArget Clone [Code No. TAK-101] can be applied to the TA cloning of PCR products amplified using Taq DNA polymerase, Blend Taq [Code No. BTQ-101], Blend Taq -Plus- [Code No. BTQ-201] or KOD Dash [Code No. LDP-101]. Almost all PCR products having dA overhanging at the 3'end are available.
High efficient TA cloning kit for KOD products
TArget Clone -Plus-
(click here)
TArget Clone -Plus- [Code No. TAK-201] can be applied to the TA cloning of blunt-end PCR products amplified using KOD -Plus- [Code No. KOD-201] or KOD FX [Code No. KFX-101]. TArget Clone -Plus- contains 10xA-attachment mix. This reagent is a mixture of anti-KOD DNA polymerase antibody specific to KOD 3'→5' exonuclease activity (proof-reading activity) and Taq DNA polymerase, which exhibits terminal transferase activity. The 10 x A-attachment mix allows for blunt end PCR products to acquire overhanging dA at the 3'-ends
TA cloning of blunt-end PCR products of KOD series
dA attachment reagent
10xA-attachment mix
(click here)Code No. TAK-301 25 reactions
10 x A-attachment mix is a reagent consists of the anti-KOD DNA polymerase antibody for the 3'→5' exonuclease activity (proof-reading activity) of the enzyme, and Taq DNA polymerase exhibits the terminal transferase activity. The PCR products from KOD -Plus- [Code No. KOD-201] and KOD FX [Code No. KFX-101] have blunt ends due to the 3'→5' exonuclease activity of KOD DNA polymerase. 10 x A-attachment mix allows the PCR products to acquire overhanging dA at the 3'-ends. The products having 3'-dA overhang can be directly cloned into arbitrary T-vectors using ligation reagents such as Ligation high Ver.2 [Code No. LGK-201].
Site-directed Mutagenesis
Site-directed Mutagenesis kit
KOD -Plus- Mutagenesis Kit
(click here)Code No. SMK-101 20 reactions
KOD -Plus- Mutagenesis Kit is an inverse PCR (iPCR)-based site-directed Mutagenesis kit using KOD DNA polymerase as a high-fidelity PCR enzyme. This reagent was developed based on a highly efficient PCR reagent, "KOD-Plus- (Code No. KOD-201)", which consists of KOD DNA polymerase and anti-KOD DNA polymerase antibodies for Hot Start PCR. This kit enables not only the introduction of point mutations, but also the introduction of large insertions and deletions. The PCR fidelity of KOD-Plus- is greater than Taq DNA polymerase (ca. 80-fold); therefore, unexpected, 2nd-site mutations can be reduced. PCR reactions can be performed using standard PCR primers and do not require phosphorylated primers, because this protocol contains a 'Phosphorylation Step' of PCR products.
Southern & Northern hybridization
High efficient hybridization solution
PerfectHyb
hybridization solution
(click here)Code No. HYB-101 250 ml
PerfectHyb hybridization solution contains an accelerator that improves the hybridization efficiency and background. The hybridization rate is accelerated and background hybridization minimized. The solution facilitates Southern and Northern blotting analysis.
100 bp DNA Ladder Marker
100 bp DNA Ladder
(click here)Code No. DNA-035 15 µg (100 gel lanes)s
DNA-135 15 µg (100 gel lanes) Loading Quick
A 100 bp DNA Ladder is suitable for use as a molecular weight standard (100 bp-1,500 bp) for agarose gel electrophoresis. A 500 bp fragment has increased intensity as a reference band. Dye-attached type and dye-mixed type (Loading Quick®) are available.
1 kb DNA Ladder Marker
1 kb DNA Ladder
(click here)Code No. DNA-032 90 µg (300 gel lanes)
A 1kb DNA Ladder is suitable for use as a molecular weight standard (1,000 bp-10,000 bp) for agarose gel electrophoresis. A 5,000 bp fragment has increased intensity as a reference band.
本文关键词：Mutagenesis，由笔耕文化传播整理发布。
，本文编号：243969
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