miR-124靶向TNF-α促进猪皮下脂肪细胞分化

发布时间：2019-01-12 08:30

【摘要】：前期研究中采用双萤光素酶报告基因验证了mi R-124与脂解因子猪TNF-α之间的靶关系,以此为基础研究mi R-124是否影响猪皮下脂肪细胞的分化。采用mi R-124模拟物mimics和抑制物inhibitor分别转染猪脂肪前体细胞并诱导其分化成成熟脂肪细胞,检测细胞的聚脂情况,甘油及甘油三酯的含量变化,荧光定量检测脂肪细胞关键转录因子PPARγ,脂肪合成和分解的主要酶FASN和HSL基因的表达变化。结果显示,过表达mi R-124能抑制TNF-α蛋白的表达,脂肪细胞脂滴多于对照组,甘油三酯(TG)含量显著增加(P0.01),甘油含量亦显著增加(P0.05),PPARγ、FASNT和HSL的表达显著上调(P0.01);抑制细胞mi R-124的表达脂滴则较少,TG含量显著减少(P0.05),PPARγ和FASN的表达均显著下调(P0.05)。mi R-124可能通过抑制TNF-α调节猪脂肪细胞的分化,为后续研究mi R-124调节脂肪代谢的相关机制奠定基础。
[Abstract]:In previous studies, the target relationship between mi R-124 and porcine TNF- 伪 was verified by using double luciferase reporter gene, and the effect of mi R-124 on the differentiation of porcine subcutaneous adipocytes was studied. Porcine adipocytes were transfected into porcine adipocytes by mi R-124 mimics and inhibitor, respectively, and induced to differentiate into mature adipocytes. The content of glycerol and triglyceride was measured. The expression of PPAR 纬, the main enzyme of fat synthesis and decomposition, FASN and HSL were detected quantitatively by fluorescence. The results showed that overexpression of mi R-124 could inhibit the expression of TNF- 伪 protein, lipid droplets in adipocytes were more than those in control group, the content of triglyceride (TG) was significantly increased (P0.01), and the content of glycerol was significantly increased (P0.05), PPAR 纬). The expression of FASNT and HSL was significantly up-regulated (P0.01). However, the expression of mi R-124 was less and the content of TG was significantly decreased (P0.05), PPAR 纬 and FASN down-regulated by P05). Mi R-124, P05). Mi R-124 may regulate the differentiation of porcine adipocytes by inhibiting TNF- 伪. It will lay a foundation for further study on the mechanism of mi R-124 regulating fat metabolism.
【作者单位】：龙岩学院生命科学学院动物营养科研创新团队;华南农业大学广东省农业动物基因组学与分子育种重点实验室;
【基金】：福建省大学生创新创业训练计划项目(201511312055) 福建省自然科学基金项目(2014J05043) 龙岩学院博士启动基金项目(LB2013012)
【分类号】：R-33

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