MiR-21协同BMP9促进间充质干细胞C3H10T1/2成骨分化

发布时间：2021-11-26 20:17

　　目的:探讨miR-21与BMP9之间的关系,明确miR-21在BMP9诱导间充质干细胞成骨分化中的作用。方法:（1）Ad-BMP9感染C3H10T1/2细胞,Real-time-PCR检测miR-21表达。RTPCR检测ALP的表达。（2）MiR-21转染C3H10T1/2细胞,Real-time-PCR检测miR-21和BMP9表达。（3）MiR-21和BMP9-CM处理C3H10 T1/2细胞,ALP活性和染色实验检测C3H10 T1/2细胞早期成骨能力。茜素红S染色实验检测钙盐沉积情况。（4）MiR-21和BMP9-CM处理C3H10T1/2细胞,Real-time-PCR检测成骨分化相关因子ALP,OCN的表达。（5）MiR-21和BMP9-CM处理C3H10T1/2细胞,Western blot检测p-Smad1/5蛋白水平的表达。结果:（1）BMP9暂时降低miR-21的表达。MiR-21也可以暂时降低BMP9的表达。（2）MiR-21可以协同BMP9增强ALP和钙盐沉积。（3）MiR-21协同BMP9增加了p-Smad1/5蛋白水平的表达。结论:MiR-21与BMP9存在... 

【文章来源】：中国生物工程杂志. 2016,36(02)北大核心CSCD

【文章页数】：8 页

【部分图文】：

miＲ-21在BMP9诱导C3H10T1/2细胞成骨分化中的表达Fig．1ThetemporarydownregulationofmiＲ-21duringBMP9-inducedosteogenicdifferentiationofC3H10T1/2cell(a)Ad-BMP9andAd-GFPcaninfectC3H10T1/2cell(×100)(b)ExpressionofALPdetectedbyＲT-PCＲ(c)ExpressionofmiＲ-21＊＊＊＊＊

2016，36(2)宋琪玲等:MiＲ-21协同BMP9促进间充质干细胞C3H10T1/2成骨分化图2过表达miＲ-21，BMP9在C3H10T1/2细胞中的表达变化Fig．2ThetemporarydownregulationofBMP9mＲNAbytransferringmiＲ-21intoC3H10T1/2cell(a)ExpressionofmiＲ-21detectedbyＲeal-timePCＲ＊＊＊P＜0．001comparedwithNCgroup(b)ExpressionofBMP9at1daydetectedbyＲeal-timePCＲ＊＊P＜0．01comparedwithNCgroup(c)ExpressionofBMP9at5daydetectedbyＲeal-timePCＲ*P＜0．5comparedwithNCgroup图3miＲ-21促进了BMP9诱导的C3H10T1/2细胞早期成骨分化Fig．3OverexpressionmiＲ-21enhancesBMP9-inducedearlyosteogenicdifferentiantionofC3H10T1/2cell(a)ALPstainingresultswererecordedinhighermagnifications(×100)(b)ALPstainingresultswererecordedinlowermagnifications(c)ALPactivitydeterminedbyALPquantitativeassay*P＜0．5comparedwithNC+BMP9group;＊＊P＜0．01comparedwithmiＲ-21group25

2016，36(2)宋琪玲等:MiＲ-21协同BMP9促进间充质干细胞C3H10T1/2成骨分化图2过表达miＲ-21，BMP9在C3H10T1/2细胞中的表达变化Fig．2ThetemporarydownregulationofBMP9mＲNAbytransferringmiＲ-21intoC3H10T1/2cell(a)ExpressionofmiＲ-21detectedbyＲeal-timePCＲ＊＊＊P＜0．001comparedwithNCgroup(b)ExpressionofBMP9at1daydetectedbyＲeal-timePCＲ＊＊P＜0．01comparedwithNCgroup(c)ExpressionofBMP9at5daydetectedbyＲeal-timePCＲ*P＜0．5comparedwithNCgroup图3miＲ-21促进了BMP9诱导的C3H10T1/2细胞早期成骨分化Fig．3OverexpressionmiＲ-21enhancesBMP9-inducedearlyosteogenicdifferentiantionofC3H10T1/2cell(a)ALPstainingresultswererecordedinhighermagnifications(×100)(b)ALPstainingresultswererecordedinlowermagnifications(c)ALPactivitydeterminedbyALPquantitativeassay*P＜0．5comparedwithNC+BMP9group;＊＊P＜0．01comparedwithmiＲ-21group25

【参考文献】：
期刊论文
[1]TGF-β/BMP signaling and other molecular events: regulation of osteoblastogenesis and bone formation[J]. Md Shaifur Rahman,Naznin Akhtar,Hossen Mohammad Jamil,Rajat Suvra Banik,Sikder M Asaduzzaman.  Bone Research. 2015(01)
[2]Spry1在miR-21调控人骨髓间充质干细胞成骨分化中的作用[J]. 杨楠,周威,王光,丁寅,金岩.  中国组织工程研究. 2014(32)
[3]miR-30a抑制BMP9诱导间充质干细胞C3H10T1/2的成骨分化[J]. 李宝林,白慧丽,张汝益,严树涓,何方,杨丹丹,刘晨,施琼.  中国生物工程杂志. 2013(11)



本文编号：3520853