七氟烷预处理联合后处理对肺缺血再灌注损伤大鼠的保护作用及机制

发布时间：2017-12-27 04:10

本文关键词：七氟烷预处理联合后处理对肺缺血再灌注损伤大鼠的保护作用及机制　出处：《山东医药》2015年17期 　论文类型：期刊论文

【摘要】：目的探讨七氟烷预处理联合后处理对大鼠肺缺血再灌注损伤(IRI)的保护作用及其机制。方法选择成年雄性SD大鼠54只,采用随机数字表法将其分为3组:假手术组(S组)仅游离左肺门,不阻断;肺缺血再灌注组(I/R组)采用阻断左肺门45 min后再灌注120 min的方法制备IRI模型;七氟烷预处理联合后处理组(SP组)阻断左肺门前吸入2.1%七氟烷,阻断左肺门45 min,于松开血管夹即刻再次吸入2.1%七氟烷30 min制备IRI模型。分别于再灌注30、60和120 min时随机取6只大鼠处死,取肺组织,测定湿/干重比(W/D比),比色法检测超氧化物歧化酶(SOD)的活性,ELISA法测定TNF-α表达,RT-PCR法测定血管紧张素转化酶(ACE)mRNA表达。光镜下观察肺组织病理学检查结果。结果与S组相比,I/R组和SP组再灌注各时点肺组织W/D比、TNF-α水平及ACE mRNA表达均升高(P均0.05),SOD活性均降低(P均0.05);与I/R组比较,SP组再灌注各时点肺组织W/D比、TNF-α水平及ACE mRNA表达均降低(P均0.05),SOD活性均升高(P均0.05)。光镜下SP组肺组织病理学损伤较I/R组减轻。结论七氟烷预处理联合后处理可能通过抑制氧化应激反应及炎症反应对大鼠肺缺血再灌注损伤发挥保护作用。
[Abstract]:Objective to investigate the protective effect and mechanism of seven Fluoroalkane preconditioning combined with postconditioning on lung ischemia reperfusion injury (IRI) in rats. Methods 54 male SD rats, were randomly divided into 3 groups: sham operation group (group S) only free left hilum, not blocked; lung ischemia reperfusion group (I/R group) by blocking the left pulmonary hilum for 45 min after reperfusion 120 min preparation of IRI model; seven isoflurane preconditioning combined with postprocessing group (group SP) occlusion of the left pulmonary hilum before inhalation of 2.1% halothane in seven, occlusion of the left pulmonary hilum for 45 min, to loosen the vascular clamp immediately again seven IRI inhalation of 2.1% halothane Model 30 min preparation. At the time of reperfusion 30, 60 and 120 min, 6 rats were sacrificed, and lung tissues were taken. The lung tissue was determined. The ratio of wet to dry weight (W/D ratio) was detected. The activity of superoxide dismutase (SOD) was detected by colorimetry. The expression of TNF- alpha was detected by ELISA, and mRNA expression of angiotensinconverting enzyme (ACE) was determined by RT-PCR. The results of pathological examination of lung tissue were observed under light microscope. Results compared with S group, I/R group and SP group at each time point of reperfusion lung tissue W/D, TNF- and ACE mRNA expression levels were significantly increased (P < 0.05), the activity of SOD decreased (P < 0.05); compared with I/R group, SP group, reperfusion lung tissue at different time points W/D and TNF- levels the expression of mRNA and ACE were decreased (P 0.05), SOD activity increased (P 0.05). The pathological damage of lung tissue in group SP was less than that in group I/R under light microscope. Conclusion the combined treatment of seven Fluoroalkane preconditioning may play a protective role in the injury of lung ischemia reperfusion injury in rats by inhibiting oxidative stress reaction and inflammatory reaction.
【作者单位】：石河子大学医学院;新疆维吾尔自治区人民医院;
【基金】：国家自然科学基金资助项目(81160016)
【分类号】：R563
【正文快照】： 肺缺血再灌注损伤(IRI)多发生于肺移植、失血性休克、体外循环后,IRI发生时,肺组织缺血后的再灌注不仅不能使肺功能恢复,反而可加重肺功能障碍和结构损伤,并最终发展为急性肺损伤(ALI)或急性呼吸窘迫综合征(ARDS)[1]。肺移植时,IRI发生率高达25%[2]。IRI机制较多,其中氧自由基

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