人脐带间充质干细胞移植治疗急性肺损伤

发布时间：2018-01-05 16:20

本文关键词：人脐带间充质干细胞移植治疗急性肺损伤　出处：《中国组织工程研究》2016年50期 　论文类型：期刊论文

【摘要】：背景:研究发现,人脐带间充质干细胞可通过减轻炎症损害改善肺通气功能。目的:观察人脐带间充质干细胞移植治疗急性肺损伤的效果。方法:将30只SD大鼠随机分为3组,正常组不做任何处理;模型组、实验组通过向气管内滴注脂多糖建立急性肺损伤模型,成功造模1 h后,实验组于气管内滴注第4代人脐带间充质干细胞悬液0.1 mL(含细胞1×10~6),正常组及模型组气管内滴注等量生理盐水。细胞移植后24 h,检测血清中白细胞介素1、白细胞介素8水平,苏木精-伊红染色观察大鼠肺组织病理改变,检测大鼠肺组织干湿质量比。结果与结论:(1)与正常组比较,模型组白细胞介素1、白细胞介素8水平及肺组织干湿质量比显著升高(P0.05);与模型组比较,实验组白细胞介素1、白细胞介素8水平及肺组织干湿质量比显著降低(P0.05);(2)正常组肺泡腔结构清晰,肺泡间隔完整;模型组肺泡间隔明显增厚,肺毛细血管出现充血、水肿,肺毛细血管及肺泡腔可见大量炎性细胞浸润,部分肺泡内可见有富含蛋白质的水肿液,肺泡腔内广泛透明膜形成;实验组肺泡结构基本清晰,肺泡间隔较厚,肺间质内有红细胞及少量炎性细胞浸润漏出;(3)结果表明,人脐带间充质干细胞移植治疗急性肺损伤,可降低炎症因子水平、减轻肺损伤。
[Abstract]:Background: the study found that human umbilical cord mesenchymal stem cells can reduce inflammation and relieve the damage of pulmonary ventilation function. Objective: To observe the effect of human umbilical cord mesenchymal stem cell transplantation in the treatment of acute lung injury. Methods: 30 SD rats were randomly divided into 3 groups, normal group without any treatment; model group. The experimental group by intratracheal instillation of lipopolysaccharide to induce acute lung injury, the success of 1 h after modeling, the experimental group in intratracheal instillation of fourth human umbilical cord mesenchymal stem cell suspension (containing 0.1 mL cells 1 * 10~6), normal group and model group intratracheal instillation of normal saline. 24 h after cell transplantation, serum interleukin 1, interleukin 8, hematoxylin eosin staining to observe the pathological changes of lung tissues were detected, rat lung wet to dry weight ratio. Results and conclusion: (1) compared with the normal group, model group of interleukin 1. Interleukin 8 levels and lung Dry and wet tissue mass ratio increased significantly (P0.05); compared with the model group, the experimental group of interleukin 1, interleukin 8 levels and lung wet to dry weight ratio was significantly decreased (P0.05); (2) normal alveolar structure clear, alveolar septum; model group alveolar septum thickening, pulmonary capillary congestion, edema, pulmonary capillaries and alveolar cavity shows infiltration of inflammatory cells, some alveolar edema fluid within lysosomes is rich in protein, extensive alveolar hyaline membrane formation; experimental group alveolar structure were clear, alveolar septum thick, interstitial lung red cells and inflammatory cells (3 leakage;) results showed that human umbilical cord mesenchymal stem cell transplantation in the treatment of acute lung injury, which can decrease the level of inflammatory factors and reduce lung injury.

【作者单位】：河北医科大学附属唐山工人医院急诊内科;河北医科大学附属唐山工人医院急诊综合监护室;迁西县三屯营镇中心卫生院检验科;
【分类号】：R563
【正文快照】： 文章快速阅读:人脐带间充质干细胞移植治疗急性肺损伤的效果正常组:不做任何处理。细胞移植后24 h:模型组:建立急性肺损伤检测血清中结果表明:模型,成功造模1 h后,炎症因子水人脐带间充质干细于气管内滴生理盐水。平,苏木精-胞移植治疗急性肺伊红染色观损伤,可降低炎症因察肺

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