熊果酸抑制矽肺大鼠转化生长因子β1、白介素1的表达及其作用机制

发布时间：2018-01-09 04:13

本文关键词：熊果酸抑制矽肺大鼠转化生长因子β1、白介素1的表达及其作用机制　出处：《环境与职业医学》2016年06期 　论文类型：期刊论文

【摘要】：[目的]研究熊果酸抑制矽肺大鼠转化生长因子β1(TGF-β1)和白介素1(IL-1)的表达及其可能作用机制。[方法]80只Wistar雄性大鼠,随机分为对照组、模型组、熊果酸组、溶剂对照组,每组20只。除对照组外,其余组采用非暴露法气管内一次性注入二氧化硅(Si O2)悬液(250 mg/kg)建立动物矽肺模型。熊果酸组自注射Si O2后每天灌胃熊果酸40 mg/kg,溶剂对照组每天灌胃质量分数为0.6%的羧甲基纤维素钠溶液(10 m L/kg),对照组灌胃生理盐水(10 m L/kg),连续56 d。ELISA法检测各组大鼠血清中IL-1和TGF-β1的质量浓度(后称"含量"),免疫组织化学法和Western blot法检测肺组织磷酸化蛋白激酶B(p-AKT1)表达情况。[结果]与对照组比较,模型组和溶剂对照组大鼠IL-1和TGF-β1含量、p-AKT1表达明显增加,差异均有统计学意义(P0.05)。与模型组和溶剂对照组比较,熊果酸组大鼠IL-1和TGF-β1含量、p-AKT1表达下降,差异有统计学意义(P0.05)。四组间分别进行各时间点的IL-1、TGF-β1含量与p-AKT1线性回归分析,各时间点相关系数均r0(P0.01)。[结论]熊果酸减少TGF-β1和IL-1表达,减缓矽肺的发展进程,其作用可能与抑制p-AKT1激活有关。
[Abstract]:[Objective] to study the inhibitory effect of ursolic acid on the expression of TGF- 尾 1 and IL 1 in silicosis rats and its possible mechanism. [Methods] Eighty male Wistar rats were randomly divided into control group, model group, ursolic acid group and solvent control group with 20 rats in each group. The rest of the group were treated with unexposed intratracheal instillation of silica / SiO2 suspension (250 mg / kg). Animal silicosis model was established. Ursolic acid group was given ursolic acid 40 mg/kg per day after injection of Sio 2. The solvent control group was given 10 mL 路kg ~ (-1) sodium carboxymethyl cellulose solution with a daily weight fraction of 0.6% mL / kg, and the control group was given 10 mL / kg normal saline solution (10 mL / kg). The concentration of IL-1 and TGF- 尾 1 in serum of rats in each group was determined by 56 d.ELISA. Immunohistochemical method and Western blot method were used to detect the expression of phosphorylated protein kinase (PPKK) in lung tissue. [Results: compared with the control group, the expression of p-AKT1 in IL-1 and TGF- 尾 1 in the model group and the solvent control group was significantly increased. Compared with model group and solvent control group, the levels of IL-1 and TGF- 尾 1 in ursolic acid group decreased. The difference was statistically significant (P 0.05). Linear regression analysis between the levels of IL-1TGF- 尾 1 and p-AKT1 was carried out at each time point among the four groups. The correlation coefficients at each time point were all R0 ~ (0) P ~ (0.01) ~ 0 ~ (-1). [Conclusion ursolic acid can decrease the expression of TGF- 尾 1 and IL-1 and slow down the development of silicosis, which may be related to the inhibition of p-AKT1 activation.
【作者单位】：华北理工大学冀唐学院;华北理工大学实验动物中心;华北理工大学基础医学院;华北理工大学公共卫生学院;
【基金】：唐山市科技局科研基金项目(编号:14130262B)
【分类号】：R135.2
【正文快照】： 熊果酸是在多科植物中广泛分布的一类五环三萜类化合物,具有抗菌、抗病毒、抗氧化、保肝、防癌抗癌等多种生物学效应[1]。本课题组前期研究结果发现,熊果酸通过纠正氧化抗氧化系统失衡、抑制核因子-κB的活化、减少胶原的产生,从而延缓矽肺发生发展过程[2-3],但具体作用机制尚

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