Ag85A、Ag85B和HspX蛋白特异性细胞免疫反应对结核性胸膜炎的辅助诊断价值

发布时间：2018-01-31 18:20

本文关键词： 结核性胸膜炎抗原细胞免疫反应 IFN-γ　出处：《武汉大学学报(医学版)》2014年01期 　论文类型：期刊论文

【摘要】：目的:探讨3种结核分枝杆菌保守抗原Ag85A、Ag85B和HspX抗原特异性细胞免疫反应对结核性胸膜炎的辅助诊断价值。方法:收集我院收治的胸腔积液患者30例,根据胸水性质,分为结核性胸膜炎组20例,非结核性胸膜炎组10例。收集胸水样本,分离胸水样本中细胞成分。原核表达、纯化并鉴定结核分枝杆菌Ag85A、Ag85B、HspX蛋白。用Ag85A、Ag85B及HspX蛋白分别刺激2组患者的胸水细胞成分,通过ELISA技术检测IFN-γ表达水平并进行组间比较。结果:Ag85A蛋白刺激后,非结核性胸膜炎组IFN-γ为(15.50±2.41)ng/L,结核性胸膜炎组IFN-γ为(1 775.00±362.86)ng/L;Ag85B蛋白刺激后,非结核性胸膜炎组IFN-γ为(14.89±2.30)ng/L,结核性胸膜炎组IFN-γ为(2 442.50±418.94)ng/L;HspX蛋白刺激后,非结核性胸膜炎组IFN-γ为(14.20±2.30)ng/L,结核性胸膜炎组IFN-γ为(3 570.00±496.41)ng/L;两组比较均P0.01。结核性胸膜炎组中,不同患者对不同抗原刺激所产生的IFN-γ水平有一定差别。结论:结核性胸膜炎患者胸水中的细胞成分经Ag85A、Ag85B及HspX蛋白刺激后,IFN-γ水平均不同程度明显升高,该方法对结核性胸膜炎有一定的辅助诊断价值。
[Abstract]:Objective: to study three conservative antigens of Mycobacterium tuberculosis (Ag85A). The value of Ag85B and HspX antigen-specific cellular immunoreactivity in the diagnosis of tuberculous pleurisy methods: 30 patients with pleural effusion were collected according to the nature of pleural effusion. It was divided into tuberculous pleurisy group (n = 20) and non-tuberculous pleurisy group (n = 10). The samples of pleural effusion were collected, the cell components in pleural effusion samples were isolated, the prokaryotic expression was obtained, and the Ag85A of Mycobacterium tuberculosis was purified and identified. Ag85B HspX protein was used to stimulate the hydrothorax cell components of the two groups with Ag85An Ag85B and HspX protein, respectively. The expression of IFN- 纬 was detected by ELISA and compared among groups. IFN- 纬 was 15.50 卤2.41 ng / L in non-tuberculous pleurisy group and 1.775.00 卤362.86 ng / L in tuberculous pleurisy group. After stimulation of Ag85B protein, IFN- 纬 was 14.89 卤2.30 ng / L in non-tuberculous pleurisy group. IFN- 纬 was 244.2.50 卤418.94 ng / L in tuberculous pleurisy group. After stimulation of HspX protein, IFN- 纬 was 14.20 卤2.30 ng / L in non-tuberculous pleurisy group. IFN- 纬 in tuberculous pleurisy group was 3 570.00 卤496.41 ng / L; In tuberculous pleurisy group. The level of IFN- 纬 produced by different antigen stimulation was different in different patients. Conclusion: the cell components in pleural effusion of tuberculous pleurisy were stimulated by Ag85Agnag85B and HspX protein. The level of IFN- 纬 in tuberculous pleurisy was increased to some extent, and this method was valuable in the diagnosis of tuberculous pleurisy.
【作者单位】：武汉市结核病防治所;
【基金】：武汉市卫生局资助课题(编号:WG10B03)
【分类号】：R521.7
【正文快照】： 结核性胸膜炎是临床常见的结核病类型,其诊断方法多样,如影像学检查、胸水性状观察、胸水酶及蛋白检测、胸膜活检、结核分枝杆菌的检查及培养等。但病原学及组织病理学检查因阳性率低、周期长,有创检查等而难以普及[1,2],所以临床上往往依靠排他性诊断。随着免疫学的进展,一些

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