缺氧诱导因子-1α在肥胖哮喘大鼠肺组织中的表达及意义

发布时间：2018-02-01 12:42

本文关键词： 肥胖哮喘缺氧诱导因子-α　出处：《细胞与分子免疫学杂志》2014年12期 　论文类型：期刊论文

【摘要】：目的观察缺氧诱导因子-1α(HIF-1α)在肥胖哮喘大鼠中的表达并探讨其作用。方法将40只清洁级雄性SD大鼠随机分为正常体质量对照组(A组)、正常体质量哮喘组(B组)、肥胖对照组(C组)、肥胖哮喘组(D组)。正常体质量组大鼠给予基础饲料,肥胖组大鼠给予高能饲料以建立肥胖大鼠模型。鸡卵清蛋白(OVA)致敏、激发,建立哮喘模型。对肺泡灌洗液(BALF)中白细胞进行计数。ImagePro Plus软件测定气管壁总面积(WAt),并以基底膜周长(Pbm)标准化,结果以气管壁厚度(WAt/Pbm)表示。免疫组织化学染色检测肺组织中HIF-1α的表达。ELISA测定血清中HIF-1α的水平。采用Pearson相关分析法分析HIF-1α的表达水平与BALF中白细胞总数、气管壁厚度的相关性。结果 D组大鼠BALF中白细胞总数为(98.0±5.5)×104/mL,较A组(24.7±3.3)×104/mL、B组(87.8±7.1)×104/mL、C组(26.1±3.8)×104/mL升高(P0.05);D组大鼠WAt/Pbm为(9.91±0.56)m2/m,较A组(6.11±0.99)m2/m、C组(5.99±0.83)m2/m升高,与B组(8.60±0.53)m2/m差异无显著性意义;D组大鼠肺组织中HIF-1α的阳性细胞表达率为(19.44±0.96)%,较A组(2.19±0.91)%、B组(18.25±1.29)%、C组(2.56±0.89)%升高;D组的大鼠血清及BALF中HIF-1α浓度分别为(29.107±1.576)ng/mL、(0.511±0.011)ng/mL,较A组(19.380±1.506)ng/mL、(0.280±0.008)ng/mL,B组(23.961±1.565)ng/mL、(0.397±0.011)ng/mL,C组(20.782±2.034)ng/mL、(0.281±0.010)ng/mL升高;HIF-1α表达水平与BALF中白细胞总数、WAt/Pbm呈正相关。结论HIF-1α在肥胖哮喘大鼠血清及肺组织中的表达明显增加,与BALF中白细胞总数、气管壁厚度呈正相关。
[Abstract]:Objective to investigate the expression of hypoxia-inducible factor-1 伪 (HIF-1 伪) in obese asthmatic rats. Methods Forty clean male SD rats were randomly divided into normal body mass control group (group A). . Normal body mass asthma group (group B), obesity control group (group C) and obese asthma group (group D) were given basic diet. Obesity group rats were given high-energy diet to establish obese rat model. Chicken ovalbumin (OVA) was sensitized and stimulated. A model of asthma was established. The white blood cells in bronchoalveolar lavage fluid (BALF) were counted. The total area of trachea wall was measured by image Pro Plus software. And the basement membrane circumference was standardized by PBM. Results based on the thickness of the gas wall, Wat / Pbmm). Immunohistochemical staining for the expression of HIF-1 伪 in lung tissue. Elisa for the determination of serum HIF-1 伪 level. Pearson correlation analysis was used to analyze the expression of HIF-1 伪 in lung tissue. The expression level and the total number of leukocytes in BALF. Results the total number of white blood cells in BALF of group D was 98.0 卤5.5 脳 10 4 / mL, which was higher than that of group A (24.7 卤3.3) 脳 10 4 / mL. Group B (87.8 卤7.1) 脳 10 ~ (4) 路m ~ (-1) 路L ~ (-1) C group (26.1 卤3.8) 脳 10 ~ (4) / mL increased P 0.05; The WAt/Pbm of group D was 9.91 卤0.56 m-2 / m, which was higher than that of group A (6.11 卤0.99m2m-2 / m) and group C (5.99 卤0.83m-2 / m). There was no significant difference between group B and group B (8.60 卤0.53m-2 / m). The positive expression rate of HIF-1 伪 in group D was 19.44 卤0.96%, which was higher than that in group A (2.19 卤0.91) and group B (18.25 卤1.29%). In group C, 2.56 卤0.89% increased. The concentration of HIF-1 伪 in serum and BALF in group D was 29.107 卤1.576ng / mL and 0.511 卤0.011ng / mL, respectively. Compared with group A (19.380 卤1.506ng / mL), group B (23.961 卤1.565ng / mL) was 0.280 卤0.008ng / mLng-1 / mL. In group C, 0.397 卤0.011 ng / mLng / mL increased in 20.782 卤2.034 ng / mL-1 / mL, 0.281 卤0.010 ng / mL; There was a positive correlation between the expression of HIF-1 伪 and Wat / PBM in BALF. Conclusion the expression of HIF-1 伪 in serum and lung tissue of obese asthmatic rats was significantly increased. There was a positive correlation with the total number of white blood cells and the thickness of trachea wall in BALF.
【作者单位】：徐州医学院附属医院呼吸内科;大屯煤电集团责任(有限)公司中心医院呼吸科;
【分类号】：R562.25
【正文快照】： 缺氧诱导因子-1α(hypoxia inducible factor 1α,HIF-1α)是近年来发现的普遍存在于哺乳动物细胞内的缺氧应答调控因子,在支气管哮喘信号转导中发挥重要作用[1-2]。目前,肥胖是一个已经具有流行性的逐渐增加的全球性难题,它与许多慢性疾病的发病率增加有关,其中哮喘的发病及

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