中枢BDNF对咳嗽变异性哮喘豚鼠模型的调控作用

发布时间：2018-02-10 20:10

本文关键词： 咳嗽变异性哮喘(CVA) 脑源性神经营养因子(BDNF) 生长相关蛋白(GAP-) 豚鼠　出处：《神经解剖学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:研究脑源性神经营养因子(BDNF)在豚鼠咳嗽变异性哮喘(CVA)发生发展中的调控机制和对生长相关蛋白43(GAP-43)表达的影响。方法:选择Hartley雄性豚鼠为研究对象,制备并鉴定CVA豚鼠模型,确定CVA发作时脑内BDNF含量和(GAP-43)表达的变化。人为改变CVA豚鼠中枢BDNF含量,观察外周气道炎症和中枢GAP-43表达的相应改变。结果:制备的CVA豚鼠模型通过鉴定符合要求。(1)模型组豚鼠中枢BDNF和GAP-43表达量明显高于生理盐水对照组(P0.05)。(2)与中枢注射人工脑脊液的CVA豚鼠相比,注射外源性BDNF可以增加肺组织气道周围的物质P免疫阳性反应物并加剧外周气道炎症,同时中枢GAP-43免疫阳性反应物分布增多(154.3±25.7 vs 78.2±22.8,P0.01)。结论:中枢BDNF在豚鼠CVA发生发展中具有调控作用,这种作用可能通过调节GAP-43表达产生。
[Abstract]:Objective: to study the regulatory mechanism of brain-derived neurotrophic factor (BDNF) in the pathogenesis and development of guinea pig cough variant asthma (CVA) and its effect on the expression of growth-associated protein 43 (GAP-43). Methods: male guinea pigs with Hartley were selected as subjects. To prepare and identify the CVA guinea pig model and determine the changes of BDNF content and GAP-43 expression in the brain during the attack of CVA. Results: the expression of BDNF and GAP-43 in guinea pigs in the model group was significantly higher than that in the normal saline control group (P 0.05) and central injection. Results: the expression of BDNF and GAP-43 in the guinea pig model was higher than that in the normal saline control group (P 0.05) and the expression of GAP-43 was higher than that in the normal saline control group (P 0.05). Compared with CVA guinea pigs with artificial cerebrospinal fluid, Injection of exogenous BDNF can increase the substance P immunoreactive substance around the airway of lung tissue and aggravate the peripheral airway inflammation. At the same time, the distribution of GAP-43 immunoreactive substances in central nervous system increased 154.3 卤25.7 vs 78.2 卤22.8P0.01.Conclusion: central BDNF plays a regulatory role in the pathogenesis and development of CVA in guinea pigs, which may be produced by regulating the expression of GAP-43.
【作者单位】：第四军医大学唐都医院重症监护室;第四军医大学唐都医院神经外科重症监护室;
【基金】：国家自然科学基金(30872444)
【分类号】：R-332;R562.25

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