ADAM33基因Q-1、T1位点多态性与黔北地区汉族COPD的相关性

发布时间：2018-02-12 22:42

本文关键词： ADAM基因多态性黔北地区汉族 COPD　出处：《实用医学杂志》2017年14期 　论文类型：期刊论文

【摘要】：目的检测ADAM33基因Q-1、T1位点多态性在慢性阻塞性肺疾病(COPD)患者中的分布频率,探讨Q-1、T1位点多态性与黔北地区汉族COPD的相关性,为COPD的预防及治疗提供理论依据。方法采用聚合酶链反应技术、电泳分离及DNA测序的方法检测ADAM33基因Q-1、T1位点多态性。结果ADAM33基因Q-1、T1位点病例组和对照组的基因型分布均符合Hardy-Weinberg平衡定律,AD-AM33基因Q-1、T1位点均有C、T等位基因。在病例组、对照组及COPD+慢性呼吸衰竭、COPD+低氧血症中基因型及等位基因频率分布差异均无统计学意义(P0.05),但T1(83 bp、112 bp)处两个杂合子在同一个样本中同时出现的概率较高(18/19),位于编码区。结论 ADAM33基因Q-1、T1位点多态性与黔北地区汉族COPD尚无相关性。
[Abstract]:Objective to detect the frequency of Q-1T 1 polymorphism of ADAM33 gene in patients with chronic obstructive pulmonary disease (COPD), and to explore the relationship between Q-1T 1 polymorphism and COPD in Han nationality in northern Guizhou. Methods Polymerase chain reaction (PCR) was used to provide theoretical basis for the prevention and treatment of COPD. Electrophoretic separation and DNA sequencing were used to detect the polymorphism of Q-1T 1 locus of ADAM33 gene. Results the genotype distribution of Q-1T 1 locus of ADAM33 gene and control group were in accordance with the Hardy-Weinberg equilibrium law. There was no significant difference in genotype and allele frequency distribution between the control group and COPD patients with chronic respiratory failure, but the probability of two heterozygotes appearing simultaneously in the same sample was higher than that in the same sample. Conclusion there is no correlation between Q 1 T 1 T 1 polymorphism of ADAM33 gene and COPD in Han nationality in northern Guizhou.
【作者单位】：遵义医学院公共卫生学院;
【基金】：国家自然科学基金(编号:81360419,81560527) 贵州省科技厅社会发展攻关项目(编号:[黔科合SY字(2013)3027;黔科合SY字(2012)3126])
【分类号】：R563.9

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